Effects of super-hard rice bread blended with black rice bran on amyloid β peptide production and abrupt increase in postprandial blood glucose levels in mice

<p>Alzheimer’s disease and type 2 diabetes are very serious diseases with the latter having been suggested to cause the former. We prepared super-hard rice bread blended with black rice bran (SRBBB), which contained a high amount of resistant starch that showed strong inhibitory activities against β-secretase and acetylcholinesterase even after heating. Black rice bran showed greater β-secretase inhibitory activity (3.6-fold) than Koshihikari rice. The bran contained more oleic acid and anthocyanin, meaning that it is potentially a biofunctional food with a high antioxidant capacity. Furthermore, aged mice, which were fed a SRBBB diet for four weeks, showed lower amyloid <i>β</i> 40 peptide in the blood than mice fed a commercial diet (<i>p</i> < 0.01). Additionally, their initial blood glucose levels (BGLs) after 12 weeks of being fed SRBBB were significantly lower than those in the control group. Taken together, our results indicate SRBBB shows promise for inhibiting not only amyloid <i>β</i> production, but also abrupt increases in postprandial BGLs.</p> <p>Characterization of amyloid β-40 protein species with ELISA method.</p

Evaluation of yield and tuber functionality in short-term cultivation of Chinese artichoke using propagated cuttings

The Chinese artichoke (Stachys affinis) is a vegetable that is also used as a medicinal plant. There are two major problems in growing Chinese artichoke. One is the re-infection of viruses when propagating virus-free seed tubers, and the other is too long a cultivation period before harvest. The application of rooted cuttings technology to tuber production could allow virus-free cuttings to be propagated annually and planted at any time of the year. We showed that 100% of the cuttings were rooted within 14 days when the electrical conductivity of the nutrient solution was less than 0.42 dS·m−1. There was no significant difference in yield or tuber size between tubers derived from rooted cuttings and those derived from seed tubers (the conventional method of cultivation). Although the yield of rooted cuttings planted in July, August and September decreased slightly with delayed planting time, tubers of marketable size were harvested even when planted in September, the shortest growing season. The stachyose content, polyphenol content and antioxidant activity of the tubers did not differ significantly with planting time. In conclusion, short-term cultivation of Chinese artichoke using rooted cuttings is feasible for commercial tuber production.</p