Nickel-Catalyzed Intermolecular [2 + 2] Cycloaddition of Conjugated Enynes with Alkenes

A nickel-catalyzed intermolecular [2 + 2] cycloaddition of conjugated enynes with alkenes has been developed. A variety of electron-deficient alkenes as well as electronically neutral norbornene and 1-decene were applicable to this reaction. The use of conjugated enynes circumvented possible side rections, such as oligomerizations and cyclotrimerizations. The isolation of reaction intermediate complexes revealed that the η³-butadienyl coordination is the key for the selective formation of cyclobutene

ChIP analysis of yeast cells treated with the exogenous NO.

<p><i>S. cerivisiae</i> BY4741 and BY4741MAC1-TAP cells grown in SD medium with/without NO treatment or in LCM medium, which is the same as SD medium without supplementation of copper, were subjected to ChIP analysis. (A) Electrophoresis of amplified DNA corresponding to the promoter region of each gene. The <i>CMD1</i> gene, which is not under the control of Mac1, was used as a negative control. (B) Quantification of DNA binding to Mac1 by densitometry. The signal from immunoprecipitated DNA was divided by the signal from the corresponding input DNA. The resultant values were normalized as the value in SD medium is 100%.</p

The activity of Cu,Zn-superoxide disumutase Sod1.

<p>Sod1 activity in <i>S. cerevisiae</i> L5685 or L5685<i>Δmac1</i> cells was measured before or after exposure to 39°C in the absence (A) or presence (B) of 100 mM NAME. The values are the means and standard deviations of three independent experiments. *<i>p</i><0.05 by Student's <i>t</i> test.</p

Intracellular copper content.

<p>The intracellular copper content in <i>S. cerevisiae</i> L5685 or L5685<i>Δmac1</i> cells was measured before or after exposure to 39°C in the absence (A) or presence (B) of 100 mM NAME. The values are the means and standard deviations of three independent experiments. *<i>p</i><0.05 by Student's <i>t</i> test.</p

Cell viability under high-temperature stress conditions.

<p>Viability of <i>S. cerevisiae</i> L5685 or L5685<i>Δmac1</i> cells was measured after exposure to high temperature in the presence (100 mM) or absence of NAME. Cell viability was expressed as percentages, calculated as follows: (no. of colonies after exposure to high temperature stress (39°C))/(no. of colonies after exposure to non-stress condition (25°C))×100. The values are the means and standard deviations of at least two independent experiments.</p

Pharmacokinetics and Safety of Triple Therapy with Vonoprazan, Amoxicillin, and Clarithromycin or Metronidazole: A Phase 1, Open-Label, Randomized, Crossover Study

<p><b>Article full text</b></p> <p><br></p> <p>The full text of this article can be found here<b>. </b><a href="https://link.springer.com/article/10.1007/s12325-016-0374-x">https://link.springer.com/article/10.1007/s12325-016-0374-x</a></p><p></p> <p><br></p> <p><b>Provide enhanced content for this article</b></p> <p><br></p> <p>If you are an author of this publication and would like to provide additional enhanced content for your article then please contact <a href="http://www.medengine.com/Redeem/”mailto:[email protected]”"><b>[email protected]</b></a>.</p> <p><br></p> <p>The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.</p> <p><br></p> <p>Other enhanced features include, but are not limited to:</p> <p><br></p> <p>• Slide decks</p> <p>• Videos and animations</p> <p>• Audio abstracts</p> <p>• Audio slides</p

RT qPCR under high-temperature stress conditions.

<p><i>S. cerevisiae</i> L5685 or L5685<i>Δmac1</i> cells were incubated at 39°C in the presence (100 mM) or absence of NAME followed by the process for RT qPCR analysis. The mRNA level of each gene was normalized to that of <i>ALG9</i> and the relative induction fold compared to cells incubated at 25°C was shown. The values are the means and standard deviations of four independent experiments. ***<i>p</i><0.001; **<i>p</i><0.01; *<i>p</i><0.05 by Student's <i>t</i> test.</p

A proposed model for the antioxidative mechanism through Mac1 activated by NO.

<p>When yeast cells are exposed to high-temperature stress, NO is produced from arginine by NOS-like activity. NO-mediated Mac1 activation through its post-translational modification upregulates the expression of <i>CTR1</i>, which encodes a copper transporter. Protein name: Fre1, cupric reductase; Ctr1, copper ion transporter; Sod1, Cu,Zn-superoxide dismutase; Ccs1, copper chaperone; Mac1, transcription factor responsible for copper metabolism.</p

RT qPCR analysis of yeast cells treated with the exogenous NO.

<p><i>S. cerevisiae</i> BY4741 or BY4741<i>Δmac1</i> cells treated or untreated with SNAP were subjected to RT qPCR analysis. The mRNA level of each gene was normalized to that of <i>ACT1</i> and the relative induction fold compared to untreated cells was shown. The values are the means and standard deviations of three independent experiments. *<i>p</i><0.05 by Student's <i>t</i> test.</p

Nickel(0)/<i>N</i>‑Heterocyclic Carbene-Catalyzed Asymmetric [2 + 2 + 2] Cycloaddition of Two Enones and an Alkyne: Access to Cyclohexenes with Four Contiguous Stereogenic Centers

A nickel(0)/chiral <i>N</i>-heterocyclic carbene (NHC)-catalyzed fully intermolecular, enantioselective [2 + 2 + 2] cycloaddition of two enones and an alkyne has been developed to access enantioenriched cyclohexenes. A single diastereomer was obtained with a successive generation of four contiguous stereogenic centers. The absolute configuration of cyclohexene derivative <b>3aa</b> was determined by X-ray diffraction and circular dichroism (CD) spectral studies