MOESM4 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

Additional file 4. Chromatographic profiles of the sugar standards in the HPAEC method. As outlined in “Methods” section, the HPAEC analyses were carried out in two separate runs using two different programs (i.e. different columns and gradients) for each sample. In bold are the sugars quantified using the respective program. (A) Program 1 was used to quantify the amounts of fucose (Fuc), rhamnose (Rha), arabinose (Ara), galactose (Gal), glucose (Glc), galacturonic acid (GalA), and glucuronic acid (GlcA) on a Dionex PA20 column eluted using a NaOH/NaOAc gradient. (B) Program 2 was used to quantify the amounts of xylose (Xyl) and mannose (Man), which eluted as one peak in program 1, on a Dionex PA1 column eluted isocratically using 2 mM NaOH

MOESM5 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

Additional file 5. Comparison of the mol% of the different types of sugars in leaf AIR from Arabidopsis, Populus, rice and switchgrass using the four different glycosyl residue composition analysis methods

L'Auto-vélo : automobilisme, cyclisme, athlétisme, yachting, aérostation, escrime, hippisme / dir. Henri Desgranges

02 avril 19121912/04/02 (A13,N4187)

MOESM6 of Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

Additional file 6. Glycosyl residue composition of (a) alcohol insoluble residue (AIR) and (b–h) wall fractions from stems of field-grown P. deltoides control and PtGAUT12.1-OE transgenic plants. Wall fractions were prepared by sequential extraction of AIR using increasingly harsh reagents: (b) 50 mM ammonium oxalate, (c) 50 mM Na2CO3, (d) 1 M KOH, (e) 4 M KOH, (f) 100 mM sodium chlorite (chlorite) and (g) 4 M KOH post-chlorite (4 M KOH PC). (h) The insoluble pellet remaining after all the extractions. Glycosyl residue composition was determined by GC–MS of trimetylsilyl (TMS) derivatives. Data are mean ± SE of three biological and two technical replicates, n = 5. *P < 0.05, **P < 0.001

MOESM4 of Development and use of a switchgrass (Panicum virgatum L.) transformation pipeline by the BioEnergy Science Center to evaluate plants for reduced cell wall recalcitrance

Additional file 4. Diagram illustrating position of three RNAi construct sequences used to silence HCTs in switchgrass. Sequences amplified and expressed in switchgrass to silence HCT1 (HCT1RNAi, purple box), HCT2 (HCT2RNAi, red box), and HCT 1 and 2 together (HCT1/2RNAi, blue box) are shown within the aligned PvHCT sequences. Areas with identity between the sequences are highlighted