Data_Sheet_1_Establishment of a triplex TaqMan quantitative real-time PCR assay for simultaneous detection of Cymbidium mosaic virus, Odontoglossum ringspot virus and Cymbidium ringspot virus.docx

Orchids are significant ornamental plants whose viral infection results in substantial economic damage. Cymbidium mosaic virus (CymMV), Odontoglossum ringspot virus (ORSV), and Cymbidium ringspot virus (CymRSV) represent three important and prevalent orchid viruses. The detection system proposed in this study uses a triplex TaqMan quantitative real-time PCR assay to identify CymMV, ORSV, and CymRSV in a simultaneous manner. We designed specific primers and probes for CymMV, ORSV, and CymRSV, with amplified sequences of 156 bp, 148 bp, and 145 bp, respectively. The minimum detection limit of the triplex qRT-PCR assay for CymMV and CymRSV was 1 copy/assay, and the minimum detection limit was 10 copies/assay for ORSV. The minimum stable detection limits for CymMV, ORSV, and CymRSV were 10, 102, and 102 copies/assay, respectively. Therefore, this system exhibited higher sensitivity (approximately 10 to 104-fold) than RT–PCR. The intra-and interassay CVs of Cq values are less than 0.55 and 0.95%, respectively, indicating that the triplex assay is highly reliable and accurate. In addition, 66 samples from five different orchid genera were analyzed using the established assay and gene chip. The detection results demonstrated that the triplex probe qRT–PCR demonstrated higher sensitivity than the gene chip, indicating that the triplex real-time PCR assay could be used for the detection of field samples. Our findings suggest that the triplex real-time RT–PCR detection system represents a rapid, simple, and accurate tool for detecting CymMV, ORSV, and CymRSV on orchids.</p

Enhanced Thermally Conductive and Microwave Absorbing Properties of Polymethyl Methacrylate/Ni@GNP Nanocomposites

In this work, Ni@GNP nanohybrids fabricated via an in situ reduction method were incorporated into polymethyl methacrylate (PMMA) to prepare PMMA/Ni@GNP nanocomposites via solution blending and hot-pressing. The Ni nanoparticles with an average diameter of 70–80 nm were observed to successfully grow on the GNP surface via SEM and TEM. Both thermally conductive and microwave absorbing properties of the prepared composites were intensively investigated. A reflection loss test experimentally suggested that the PMMA/Ni@GNP composites showed a good microwave absorbing property, among which the composite containing 30 wt % Ni@GNP displayed the best microwave absorption performance (with a value of −30 dB for RLmin in the C band). The thermal conduction behavior of the composites was jointly studied by infrared thermal imaging (ITI) and enthalpy transformation method together with a four-parameter model. The average heating rate during an initial stage was obviously found to increase from 65.4 to 103.2 °C/min, indicating that a well-defined heat conduction network gradually formed in the composites, which exerted positive influence on heat conduction. The composites generally bore perfect thermal conductivities (e.g., with a maximum in-plane thermal conductivity of 9.02 W/m·K and a maximum out-of-plane value of 1.29 W/m·K), and the variations of thermal conductivity versus filler loading should be attributed to the construction of an anisotropically thermally conductive network in the composites. The PMMA/Ni@GNP composites with desirable microwave absorption and thermal conductivity would find potential applications in 5G communication devices