Additional file 1 of High genetic diversity and different type VI secretion systems in Enterobacter species revealed by comparative genomics analysis

Additional file 1. Similarity identity matrix of average amino acid identity (AAI) (upper triangle) and average nucleotide identity (ANI) (lower triangle) among Enterobacter species. The color represents the numerical size, red color means the highest value, green color represents the lowest value.Strains are considered one species when share >95% AAI and ANI

Additional file 5 of High genetic diversity and different type VI secretion systems in Enterobacter species revealed by comparative genomics analysis

Additional file 5. BLAST comparison and taxonomic analysis based on PAAR domain-containing protein in T6SS-C gene cluster. The phylogenetic tree is inferred using the Neighbor-Joining method

Additional file 3 of High genetic diversity and different type VI secretion systems in Enterobacter species revealed by comparative genomics analysis

Additional file 3. Pan-genome, core-genome and singleton development of different Enterobacter species

Additional file 4 of High genetic diversity and different type VI secretion systems in Enterobacter species revealed by comparative genomics analysis

Additional file 4. Prediction of conserved domain in T6SS PAAR-repeat proteins (only list the protein with N-terminal PAAR motif and a C-terminal toxin domain)

Additional file 2 of High genetic diversity and different type VI secretion systems in Enterobacter species revealed by comparative genomics analysis

Additional file 2. Pan-genome features in different Enterobacter species

Additional file 1 of First-trimester fetal size, accelerated growth in utero, and child neurodevelopment in a cohort study

Supplementary Material 1

Raw image data to support Fig 2.

Correction: Suppression of Mitochondrial Complex I Influences Cell Metastatic Properties</p

Knockdown of GRIM-19 and NDUFS3 Suppresses Mitochondrial Complex I Activity.

<p>The knockdown efficiency of <i>GRIM-19</i> or <i>NDUFS3</i> was assessed by densitometric analysis of GRIM-19 and NDUFS3 bands on western blot using GAPDH as loading control (A). The complex I activity was tested by measuring absorbance at a wavelength of 340 nm using spectrophotometer with NADH as the substrate. The rotenone-sensitive NADH oxidation rate which represents the complex I activity was calculated by subtracting the NADH oxidation rate in the presence of rotenone from the total NADH oxidation rate in the absence of rotenone (B). Asterisks indicate a p-value of ≤0.05 (*) as determined by Student's T-test.</p

Partial Inhibition of Complex I by Knockdown <i>GRIM-19</i>, <i>NDUFS3</i> or by Chemical Inhibitor Increased ROS Generation thereby Increasing the FN and N-cadherin Levels.

<p>Knockdown of GRIM-19 or NDUFS3 increases ROS generation as compared to the SC or WT cells. ROS was measured by staining cells with H₂DCFH-DA followed by FACS scan (A). Rotenone (RT) treatment induces the ROS production in the WT cells in a dose dependent manner (B). The effect of ROS on FN expression was assessed by Western blot after treating SC cells with rotenone (2.5 µM) in (C) or 200 µM tBHP in (D) at indicated days. The ROS scavenger NAC can decrease the FN expression level of G19 Hela cells (E).</p

Molecular Profiling of EMT-Associated Proteins.

<p>Western blot analysis of FN, its receptor integrins α5 and β1, N-cadherin, Vimentin, Mucin 1, Desmoplakin and HIF1a in WT, SC, G19 and p30 Hela cells (A). FN (B, left panel) and N-cadherin (B, right panel) expression in monolayer adhesion culture environment was assessed using immunofluorescence. Scale bar = 20 µm.</p