Making use of Capuchins’ behavioral propensities to obtain hair samples for DNA analyses

Genotyping wild and captive capuchins has become a priority and hair bulbs have high quality DNA. Here, we describe a method to non-invasively collect fresh-plucked strands of hair that exploits capuchins’ manual dexterity and propensity to grasp and extract food. The apparatus consists of a transparent tube baited with food. Its extraction requires the monkey to place its forearm in contact with double-sided tape applied on the inner surface of the tube entrance. The “tube” method, successfully implemented with captive (N=23) and wild (N=21) capuchins, allowed us to obtain hair bulbs from most individuals and usable genomic DNA was extracted even from a single bulb

Avaliação de Cultivares de Arroz em Doses Contrastante de Nitrogênio em Solos de Várzea Irrigada

Monitoring of nutrients through the soil analysis and the direct method of destruction of the plant are the most common methods for assessing nutrients for maximum crop production. One method that has been used in other countries (Japan and USA) to estimate the level of need for N and nitrogen application in rice is the portable chlorophyll meter, clorofilog CFL 1030 (Faker ®). Given that the objective of this study was to assess the level of leaf nitrogen in rice plants, to evaluate the effect of nitrogen by using indirect in rice cultivars, as well as the agronomic characteristics. We evaluated the plant height, number of epikelets per panicle, 100 grain weight, the leaf N content, chlorophyll content and grain yield. The rice cultivars are influenced by levels of nitrogen for plant height, nitrogen content, chlorophyll and 100 grain weight; cultivate the Best 2000 was what got greater productivity.O acompanhamento dos nutrientes através da análise de solo e pelo método direto de destruição da planta são os métodos mais comuns para avaliação dos nutrientes visando a máxima produção das culturas. Um método que vem sendo utilizado em outros países (Japão e EUA) para estimar o nível de N e necessidade de adubação nitrogenada em cobertura na cultura do arroz é o medidor portátil de clorofila, clorofilog CFL 1030 (FAKER®). Diante disso o objetivo desse trabalho foi de avaliar o teor de nitrogênio na folha em plantas de arroz, avaliar o efeito do nitrogênio pelo uso indireto em cultivares de arroz, bem como, as características agronômicas.  Avaliou-se a altura de plantas, o número de espigueta por panícula, a massa de cem grãos, o teor de nitrogênio foliar, o teor de clorofila e a produtividade de grão. Os cultivares de arroz são influenciados pelas doses de nitrogênio, para a altura de planta, teor de nitrogênio, clorofila e a massa de cem grãos; o cultivar Best 2000 foi o que obteve maior produtividade

Estágio extra mural como espaço de aprendizagem para abordagem interprofissional à pessoa com deficiência no SUS: um relato de experiência / Extra mural internship as a learning space for the interprofessional approach to the person with disability in the SUS: an experience report

O presente relato tem como objetivo descrever a experiência dos alunos do curso de Odontologia da Universidade de Fortaleza durante o módulo do Estágio Extra Mural em uma Instituição de cuidado a pessoas com deficiência. De caráter descritivo, esta experiência é resultado das atividades de estágio na Associação de Pais e Amigos de Excepcionais, localizada no município de Fortaleza-Ce, com o propósito de viabilizar a atenção integral e resguardar os direitos da pessoa com deficiência e a sua inclusão social. A experiência compreendeu o acompanhamento por meio da observação ativa e de diálogos informais durante os atendimentos dos profissionais de Fonoaudiologia, Psicologia, Terapia Ocupacional e Pedagogia, além das atividades educativas e preventivas relacionadas à saúde bucal e procedimentos clínicos odontológicos. O aprendizado adquirido foi de grande valia para formação acadêmica e humana dos discentes. Concluindo-se que é de extrema importância a abordagem multiprofissional no atendimento à Pessoas com Deficiência

Immunotherapy and immunochemotherapy in visceral leishmaniasis : promising treatments for this neglected disease.

Leishmaniasis has several clinical forms: self-healing or chronic cutaneous leishmaniasis or post-kala-azar dermal leishmaniasis; mucosal leishmaniasis; visceral leishmaniasis (VL), which is fatal if left untreated.The epidemiology and clinical features of VL vary greatly due to the interaction of multiple factors including parasite strains, vectors, host genetics, and the environment. Human immunodeficiency virus infection augments the severity of VL increasing the risk of developing active disease by 100?2320 times. An effective vaccine for humans is not yet available. Resistance to chemotherapy is a growing problem in many regions, and the costs associated with drug identification and development, make commercial production for leishmaniasis, unattractive.The toxicity of currently drugs, their long treatment course, and limited efficacy are significant concerns. For cutaneous disease, many studies have shown promising results with immunotherapy/immunochemotherapy, aimed to modulate and activate the immune response to obtain a therapeutic cure. Nowadays, the focus of many groups centers on treating canine VL by using vaccines and immunomodulators with or without chemotherapy. In human disease, the use of cytokines like interferon-g associated with pentavalent antimonials demonstrated promising results in patients that did not respond to conventional treatment. In mice, immunomodulation based on monoclonal antibodies to remove endogenous immunosuppressive cytokines (interleukin-10) or block their receptors, antigen-pulsed syngeneic dendritic cells, or biological products like Pam3Cys (TLR ligand) has already been shown as a prospective treatment of the disease. This review addresses VL treatment, particularly immunotherapy and/or immunochemotherapy as an alternative to conventional drug treatment in experimental models, canine VL, and human disease

Patient-physician discordance in assessment of adherence to inhaled controller medication: a cross-sectional analysis of two cohorts

We aimed to compare patient's and physician's ratings of inhaled medication adherence and to identify predictors of patient-physician discordance.(SFRH/BPD/115169/2016) funded by Fundação para a Ciência e Tecnologia (FCT); ERDF (European Regional Development Fund) through the operations: POCI-01-0145-FEDER-029130 ('mINSPIRERS—mHealth to measure and improve adherence to medication in chronic obstructive respiratory diseases—generalisation and evaluation of gamification, peer support and advanced image processing technologies') cofunded by the COMPETE2020 (Programa Operacional Competitividade e Internacionalização), Portugal 2020 and by Portuguese Funds through FCT (Fundação para a Ciência e a Tecnologia).info:eu-repo/semantics/publishedVersio