Table_1_Non-host plant odors influence the tritrophic interaction between tomato, its foliar herbivore Tuta absoluta and mirid predator Nesidiocoris tenuis.docx

The tomato leafminer, Tuta absoluta is a destructive invasive pest of cultivated tomato and other Solanaceae plants, with yield losses of 80-100%. Mirid predators are key natural enemies of T. absoluta, but they also feed on host plants in the absence of their prey. Management of T. absoluta is a challenge due to its high biotic potential, resistance to many insecticides and the absence of sufficiently adapted auxiliary fauna in its new dispersion zones. Olfaction plays an important role in the tritrophic interaction between tomato, its herbivore pest T. absoluta and its mirid predators, which can be influenced by non-host plant odors. However, how non-host odours shape this interaction is poorly understood. Previously, we had demonstrated belowground crop protection properties of certain Asteraceae plants against the root-knot nematode Meloidogyne incognita, pest of tomato and other Solanaceae plants. Additionally, Asteraceae plants impact negatively on feeding behavior of above-ground pests of Solanaceae plants, including the greenhouse whitefly (Trialeurodes vaporariorum) and green peach aphid (Myzus persicae). Here, we tested the hypothesis that foliar volatiles from some of these non-host Asteraceae plants can influence the tomato-T. absoluta-mirid predator tritrophic interaction. In olfactometer assays, T. absoluta females were attracted to volatiles of the Solanaceae host plants tomato and giant nightshade but avoided volatiles of the Asteraceae plants, blackjack and marigold, and the positive control, wild tomato, when tested alone or in combination with the host plants. Coupled gas chromatography-mass spectrometry analysis showed that host and non-host plants varied in their emission of volatiles, mainly monoterpenes and sesquiterpenes. Random forest analysis combined with behavioral assays identified monoterpenes as the host plant attractive blend to T. absoluta and its mirid predator, with sesquiterpenes identified as the non-host plant repellent blend against T. absoluta. Contrastingly, the mirid predator was indifferent to the non-host plant repellent sesquiterpenes. Our findings indicate that terpenes influence the tomato-T. absoluta-mirid predator tritrophic interaction. Further, our results emphasize the importance of studying crop protection from a holistic approach to identify companion crops that serve multi-functional roles.</p

Data_Sheet_1_Differential responses of Bactrocera dorsalis and its parasitoids to headspaces of different varieties of tree-attached mango fruits and the associated chemical profiles.PDF

Bactrocera dorsalis (Hendel) is a major pest of fruits and vegetables worldwide with documented losses of up to 100%. Various management techniques including the use of parasitoids, such as Fopius arisanus (Sonan) and Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae) within the context of the Integrated Pest Management (IPM) approach have been deployed for its control. The effectiveness of parasitoids is well understood, but knowledge of the semiochemicals that mediate their behavior, as well as that of the host fruit fly to tree-attached mangoes, is lacking. Here, we first compared the attractiveness of the above-mentioned fruit fly and its parasitoids to volatiles of different treatments (non-infested physiologically mature unripe and ripe mangoes, mangoes newly exposed to ovipositing B. dorsalis, and mangoes on day 7 and day 9 post-oviposition) of tree-attached Kent, Apple, and Haden mango varieties relative to control (clean air). The fruit fly was significantly more attracted to the mango volatiles (up to 93% of responsive insects) compared to the control (clean air). Fopius arisanus was significantly more attracted to mangoes with ovipositing fruit flies (68–76%) while D. longicaudata was significantly more attracted to day 9 post-oviposited mangoes (64–72%) compared to the control. Secondly, we elucidated the headspace volatile profiles of the non-infested and infested tree-attached mangoes using gas chromatography linked to mass spectrometry (GC-MS). The volatiles revealed various types of organic compounds with qualitative and quantitative differences. The majority of the compounds were esters making 33.8% of the total number, followed by sesquiterpenes-16.4%, and monoterpenes-15.4% among others. Most compounds had higher release rates in headspace volatiles of fruit fly-infested mangoes. Lastly, we harvested the infested mangoes and incubated them for puparia recovery. The number of puparia recovered varied according to the mango variety with Apple mango registering 81.7% of the total, while none was recovered from Kent. These results represent the first report of the changes in the headspace components of non-infested and infested tree-attached mangoes and the associated differential responses of the mentioned insects. A follow-up study can reveal whether there is a convergence in olfactomes which is significant when developing baits that selectively attract the fruit fly and not its natural enemies and fill the knowledge gap from an evolutionary ecological perspective.</p