Log₁₀ of telomere length in the lung vs. log₁₀ of telomere length in the blood.

<p>Log₁₀ of telomere length in the lung vs. log₁₀ of telomere length in the blood.</p

Demographic and genotypic characteristics of subjects from the Alpha-1 Foundation DNA and Tissue Bank and the Lung Health Study.

<p>*Mean ± standard error of the mean.</p

Log₁₀ of telomere length in α₁-antitrypsin deficient COPD patients vs. log₁₀ of telomere length in COPD controls.

<p>Log₁₀ of telomere length in α₁-antitrypsin deficient COPD patients vs. log₁₀ of telomere length in COPD controls.</p

Mean log₁₀ of telomere length in the blood and lung tissue.

<p>Mean log₁₀ of telomere length in the blood and lung tissue.</p

Association between rs1078761 genotype and BPIFA1 levels in the saliva of clinically stable patients with CF.

The y axis shows P values in the–log10 scale for polymorphisms that were tested for association in the meta-analysis of 6365 CF patients for lung disease severity. rs1078761 remained the most significant association in the region (purple diamond). The extent of linkage disequilibrium (measured by the r2 statistic) with rs1078761 for the remaining SNPs is indicated with colors.</p

Quantification of colony forming units and inflammatory cytokine production in IB3-1 cells transfected with BPIFA1 plasmid.

IB3-1 cells were transfected with empty vector, 100 ng or 500 ng of BPIFA1 plasmid, and stimulated with P. aeruginosa for 5 hours. This was followed by a) detection of secreted BPIFA1 in transfected IB3-1 cells, b) quantification of colony forming units, and c) quantification of IL-8 and IL-6 production by ELISA. Values represent mean plus standard error of the mean. The Kruskal-Wallis test was used to determine statistical differences between conditions and the corresponding P values are shown.</p

Quantification of inflammatory cytokine production by airway epithelial cells pretreated with recombinant BPIFA1 or BPIFB1 prior to bacterial stimulation.

Quantification of IL-8 and IL-6 by ELISA produced by IB3-1, CuFi-1 and CFBE41o- airway epithelial cell lines that were unstimulated, stimulated with heat-killed P. aeruginosa, pretreated with recombinant BPIFA1 for 30 min followed by stimulation with heat-killed P. aeruginosa, or pretreated with recombinant BPIFB1 for 30 min followed by stimulation with heat-killed P. aeruginosa. ANOVA was used to identify statistical differences in IL-6 and IL-8 levels between conditions with * denoting P < 0.05.</p

Results for genetic association of rs1078761 with CF lung disease severity.

Results for genetic association of rs1078761 with CF lung disease severity.</p

Locus zoom plot of the region 10 kb upstream of <i>BPIFA1</i> to 10 kb downstream of <i>BPIFB1</i>.

The y axis shows P values in the–log10 scale for polymorphisms that were tested for association in the meta-analysis of 6365 CF patients for lung disease severity. rs1078761 remained the most significant association in the region (purple diamond). The extent of linkage disequilibrium (measured by the r2 statistic) with rs1078761 for the remaining SNPs is indicated with colors.</p

<i>P</i>. <i>aeruginosa</i> growth curves and colony forming units with the addition of BPIFA1 or BPIFB1 recombinant proteins.

a) Growth curves of the PAO1 strain of P. aeruginosa treated with 1, 5 and 10 μg of recombinant BPIFA1 or 1 and 10 μg of BPIFB1 protein. b-c) Quantification of colony forming units in P. aeruginosa pretreated with recombinant BPIFA1 (b) or recombinant BPIFB1 (c). Values represent mean plus standard error of the mean. The Kruskal-Wallis test was used to assess for statistical differences in colony forming units between conditions and the corresponding P values are shown. Colony forming units were measured at 4 hours and 24 hours of bacterial culture growth.</p