Effect of Yeast Saccharomyces Cerevisiae Addition to Lactating Dairy Cows Ration Upon Milk Production and Composition

. On farm level, the price of milk is affected by its fat content. On the other hand, improving milk quality by the use of better ration economically is not feasible. The problem is how to find an appropriate, easily found, relatively cheap and efficient ration for producing better quantity and quality of milk. An experimental research was conducted using Saccharomyces cerevisiae yeast in lactating dairy cows ration, in a 4x4 Latin Square Design, with animal trial as column and trial period as row. Each trial period consisted of 14-day preliminary and 7-day trial period (collection period). The treatment to be tested were four levels of yeast addition, namely : 0, 5, 10 and 15 g/cow/day. The variables measured were daily milk production (4% FCM) and milk composition (solid non fat, fat, protein, lactose). The result showed that the addition of yeast had no significant effect on milk production and milk composition, but tended to increase milk fat in which the highest fat content of 5.13 % was achieved when 8.5 g/cow/day was suplemented. The average milk production, solid non fat, lactose, fat, and protein were 9.55 kg/day, 8.70%, 3.99%, 4.50% and 0.13%, respectively. Based on all measured variables, it can be concluded that the addition of yeast Saccharomyces cerevisiae up to 15 g/cow/day to lactating dairy cows ration did not effectively improve milk production and milk composition

Effects of Slow Release Urea Supplementation of Sheep Protein Source Feed Protected with Condensed Tannin From Leucaena on Protein Degradation in Rumen and Post-rumen in Vitro

The objectives of the research were to supply nitrogen for protein synthesis of ruminal microbe from slow release urea and to supply post-rumen protein from soybean meal protected with condensed tannin (CT) from crude Leucaena leaves extract. In Experiment 1, slow release urea (SRU) was made by extrusion of cassava waste-urea, tapioca meal-urea and cassava meal-urea. Evaluation of SRU properties was based on residual nitrogen concentration and ruminal fermentation products (total volatile fatty acid and ammonia-nitrogen) in vitro. In Experiment 2, soybean meal protected with CT from crude Leucaena leaves extract as much as 1,68 g tannin/100 g DM soybean meal. SRU that was selected from Experiment 1 was used as the supplement in sheep ration whose protein source was protected with CT. SRU supplement in basal rations was 0, 6.36, 12.75, 19.11% DM. In vitro result in Experiment 1, showed that SRU of cassava waste, tapioca meal and cassava meal were different (P<0,01) on residual nitrogen content, total VFA, ammonia-N, and fermentation time. The optimum SRU obtained from cassava waste by inhibiting nitrogen hydrolysis up to 14 h. In Experiment 2, SRU supplementation of cassava waste on basal ration whose protein source was protected with CT increased dry matter (DM) degradability (P<0,01), total VFA (P<0,01), ammonia-N (P<0,01), and did not affect crude protein (CP) ruminal degradation. Post-ruminal degradability of DM and CP increased (P<0.01) in line with the increasing supplement, and reached the optimum level at 12,75 – 19,11% DM. In conclusion, cassava waste was a potent SRU through extrusion process and could be harnessed as SRU supplement in ration with protein source protected with condensed tannin to improve ruminal microbe protein synthesis

Effect of Yeast Saccharomyces cerevisiae Addition to Lactating Dairy Cows Ration Upon Milk Production and Composition

Abstract.  On farm level, the price of milk is affected by its fat content. On the other hand, improving milk quality by the use of better ration economically is not feasible. The problem is how to find an appropriate, easily found, relatively cheap and efficient ration for producing better quantity and quality of milk. An experimental research was conducted using Saccharomyces cerevisiae yeast in lactating dairy cows ration, in a  4x4 Latin Square Design, with animal trial as column and trial period as row. Each trial period consisted of 14-day preliminary and 7-day trial period (collection period). The treatment to be tested were four levels of yeast addition, namely : 0, 5, 10 and 15 g/cow/day. The variables measured were daily milk production (4% FCM) and milk composition (solid non fat, fat, protein, lactose). The result showed that the addition of yeast had no significant effect on milk production and milk composition, but tended to increase milk fat in which the highest fat content of 5.13 % was achieved when 8.5 g/cow/day was suplemented. The average milk production, solid non fat, lactose, fat, and protein were 9.55 kg/day, 8.70%, 3.99%, 4.50% and 0.13%, respectively. Based on all measured variables, it can be concluded that the addition of yeast Saccharomyces cerevisiae up to 15 g/cow/day to lactating dairy cows ration did not effectively improve milk production and milk composition. Key words: yeast addition, milk production, milk composition. Abstrak.  Penentuan harga susu di tingkat peternak sangat dipengaruhi oleh kadar lemak susu. Perbaikan komposisi susu dengan peningkatan kualitas pakan sulit dilakukan, karena tidak ekonomis. Oleh karena itu perlu dicari ransum yang murah, mudah didapat dan efisien untuk meningkatkan produksi dan komposisi susu. Suatu penelitian telah dilakukan dengan menggunakan ragi Saccharomyces cerevisiae dalam ransum sapi perah laktasi. Penelitian dilaksanakan dengan metode eksperimental, menggunakan Rancangan Bujur Sangkar Latin 4x4. Hewan percobaan sebagai kolom dan periode percobaan sebagai lajur. Setiap periode percobaan terdiri atas 14 hari masa preliminari dan 7 hari percobaan (masa koleksi). Perlakuan yang diuji adalah 4 taraf penambahan ragi Saccharomyces cerevisiae : 0, 5, 10, dan 15 gr/ekor/hari. Peubah respon yang diamati adalah produksi susu harian (4% FCM) dan komposisi susu (BKTL=Bahan Kering Tanpa Lemak, lemak, protein dan laktosa).  Hasil penelitian menunjukkan bahwa penambahan ragi tidak berpengaruh nyata terhadap produksi 4% FCM (Fat Corrected Milk) maupun komposisi susu, tetapi cenderung meningkatkan lemak susu dan mencapai kadar tertinggi 5,13% pada penambahan ragi 8,50 g/ekor/hari. Rataan produksi susu 9,55 kg/hari, BKTL (Bahan Kering Tanpa Lemak) 8,70 %, laktosa 3,99%, lemak 4,50% dan protein 0,13%. Berdasarkan semua peubah respon yang diukur dapat disimpulkan bahwa penambahan ragi Saccharomyces cerevisiae sampai 15 gr/ekor/hari belum berhasil memperbaiki produksi dan komposisi susu. Kata kunci : penambahan ragi, produksi susu, komposisi susu

Effects of Slow Release Urea Supplementation of Sheep Protein Source Feed Protected with Condensed Tannin from Leucaena on Protein Degradation in Rumen and Post-rumen In Vitro

The objectives of the research were to supply nitrogen for protein synthesis of ruminal microbe from slow release urea and to supply post-rumen protein from soybean meal protected with condensed tannin (CT) from crude Leucaena leaves extract. In Experiment 1, slow release urea (SRU) was made by extrusion of cassava waste-urea, tapioca meal-urea and cassava meal-urea. Evaluation of SRU properties was based on residual nitrogen concentration and ruminal fermentation products (total volatile fatty acid and ammonia-nitrogen) in vitro. In Experiment 2, soybean meal protected with CT from crude Leucaena leaves extract as much as 1,68 g tannin/100 g DM soybean meal. SRU that was selected from Experiment 1 was used as the supplement in sheep ration whose protein source was protected with CT. SRU supplement in basal rations was 0, 6.36, 12.75, 19.11% DM. In vitro result in Experiment 1, showed that SRU of cassava waste, tapioca meal and cassava meal were different (P<0,01) on residual nitrogen content, total VFA, ammonia-N, and fermentation time. The optimum SRU obtained from cassava waste by inhibiting nitrogen hydrolysis up to 14 h. In Experiment 2, SRU supplementation of cassava waste on basal ration whose protein source was protected with CT increased dry matter (DM) degradability (P<0,01), total VFA (P<0,01), ammonia-N (P<0,01), and did not affect crude protein (CP) ruminal degradation. Post-ruminal degradability of DM and CP increased (P<0.01) in line with the increasing supplement, and reached the optimum level at 12,75 – 19,11% DM. In conclusion, cassava waste was a potent SRU through extrusion process and could be harnessed as SRU supplement in ration with protein source protected with condensed tannin to improve ruminal microbe protein synthesis

Pengujian Awal Penanda Jenis Spesifik Pada Jamur Yang Berpotensi Sebagai Agens Pengendali Hayati Penyakit Busuk Akar Pada Akasia

Root rot caused by Ganoderma philippii (Bres. & Henn. ex Sacc.) Bres., is an important disease in Acacia plantation. A strategy to control this disease is currently being developed, particularly on the application of biological control agent (BCA). Species specific primers for rapid identification of potential fungi as BCA were developed. This study aimed to obtain the best DNA condition and the best primers for species specific identification. DNA with 20x dilution is the best condition for amplification of the ITS fragment thus used for rapid species identification. The best primer set to detect Cerrena sp. is CrF1/CrR1, PbF2/PbR2 is the best primer set for Phlebiopsis sp. 1. There was no specific primer suitable to detect Phlebia sp. 1 and Phlebia sp. 2 only but Pl-2F1/Pl-2R4 is the best primer set for Phlebia spp

Keragaman Genetik Empat Populasi Lntsia Bijuga Berdasarkan Penanda Rapd dan Implikasinya Bagi Program Konservasi Genetik

Intsia bijuga atau merbau merupakan jenis kayu bemilai ekonomi tinggi dan telah mengalami eksploitasi yang intensif. Penelitian ini bertujuan mempelajari keragaman genetik populasi merbau guna membantu penyusunan strategi konservasi genetik, dengan menggunakan penanda RAPD. Sampel daun dikumpulkan dari 4 populasi dan dianalisa menggunakan 15 primer RAPD yang menghasilkan 77 lokus polimorfik. Rata-rata lokus polimorfik per primer adalah 5.1. Nilai keragaman genetik rerata dalam populasi sebesar 0,296 sedangkan keragaman antara populasi 0.141. Analisis klaster membagi keempat populasi menjadi dua kelompok populasi yaitu Carita dan Manokwari pada kelompok pertama, sedangkan kelompok kedua terdiri dari populasi Temate dan Nabire. Pembagian kelompok antara Manokwari dan Nabire lebih memperjelas pembagian Papua menjadi 6 wilayah geogenetik

Seleksi dan Perolehan Genetik pada Uji Keturunan Generasi Kedua Kayuputih (Melaleuca Cajuputi Subsp. Cajuputi) di Gunungkidul

Breeding for cajuput in Indonesia has moved into advanced generation breeding cycle through establishing second-generation progeny trial. A series of selection would be practiced in the trial before converting into a seedling seed orchard to produce genetically improved seed. In this study, a series of selection, followed by prediction of genetic gain will be observed in the second-generation progeny trial of cajuput established at Gunungkidul. The trial was laid out in randomized complete block design (RCBD), 65 families, three tree-plot, four replications, and spacing of 3 × 1,5 meter. Measurement was conducted on height and diameter at 2 years age. In the trial, within-plot selection has been practiced phenotypicaly by retaining one of the best tree out of the three tree within each plot. Succesive family selection was then simulated from the result of within-plot selection. Results of study showed that estimates of heritabily for diameter were higher than that for height. Genetic and phenotypic correlation between the two traits was moderate at around 0.57. Within-plot selection practiced in the trial resulted positive selection differential for all measured traits. Diameter showed higher coefficient weight of selection (0.4280) than height (0.0406) which indicates that the practiced within-plot selection was more imposed for diameter than that for height. Genetic gain from within-plot selection calculated using selection index were 20.76% for diameter and 12.73% for height. Simulated family selection using the same coefficient weight as within-plot selection resulted lower genetic gain at around 12.26% and 7.52% for diameter and height, respectivelly

Estimasi Parameter Genetik Pada Uji Keturunan Alstonia Scholaris Umur Dua Tahun Di Gunungkidul, YOGYAKARTA

Estimates of genetic parameter were observed at two years old of Alstonia scholaris progeny trial in Gunungkidul, Yogyakarta. The experimental design of the trial was laid out in Randomized Complete Block Design consisting of 48 families, 5 replications, 4 tree-plot and spacing of 4 x 2 meters. This study is aimed to observe the growth variation (height and stem diameter) and the estimates of heritability and genetic correlation among the two growth traits. The results of study showed that the tested families were significantly different for height and stem diameter. The estimate of individual heritability was high at around 0.53 and 0.44 for height and stem diameter, respectively. While the corresponding of family heritability was at around 0.67 and 0.63 for height and stem diameter, respectively. Positive and strong genetic correlation were found between height and stem diameter of this species at two years of age (rg = 0.94)

Karakteristik Pembungaan Dan Sistem Perkawinan Nyamplung (Calophyllum Inophyllum) Pada Hutan Tanaman Di Watusipat, Gunung Kidul

Flowering are influenced by internal factors, such as genetic and phytohormone, and environment factors, such as sunlight and nutrition intake. The flowering characteristics influence fruiting and genetic diversity seedlings through mating systems. This study aims to assess flowering and fruiting characteristics and to determine pattern of mating system of a Calophyllum inophyllum plantation at Watusipat, Gunung Kidul. Flowering and fruiting were observed at 4 locations, 3 parts of crown, and 4 main directions to know the effects of sunlight, nutrition intake and phytohormone in the flowering process. Mating system was assessed by comparing genetic diversity values between parent trees and offsprings. The values of genetic diversity were analyzed using 5 RAPD primers with 17 polymorphic loci. Analysis of variant showed that the locations, crown parts, directions and interaction between a location and direction significantly affected to differences number of flowers and fruits. Values of genetic diversity (h) of parent trees ranged between 0.1471 and 0.3056. The values increased at almost overall offsprings; it ranged between 0.2864 and 0.3750. Values of genetic distance (Da) between parent trees were high and very high (0.197 – 0.364), but the values was decreased between parent trees and their offspring, even between offspring populations. A dendrogram showed two main clusters; first cluster consisted parent trees at up edge with rare trees and second cluster consisted sub cluster parent trees at up edge; sub cluster parent trees at down middle; and sub cluster parent trees at down edge and overall offsprings. Flowering/ fruiting characteristics and pattern of mating systems of C. inophyllum were briefly discussed