An intelligent control method based on artifi cial neural network model

The topology structure of the artifi cial neural network is an intelligent control model, which is used for intelligent vehicle control system and household sweeping robot. When setting the intelligent control system, the connection point of each network is regarded as a neuron in the nervous system, and each connection point has input and output functions. Only when the input of nodes reaches a certain threshold can the output function of nodes be stimulated. Using the networking mode of artifi cial neural network model, the mobile node can output in multiple directions. If the input direction of a certain path is the same as that of other nodes, it can choose to avoid and choose another path. The weighted value of each path between nodes is diff erent, which means that the infl uence of the front node on the current node is diff erent. The control method based on artifi cial neural network model can be applied to vehicle control, household sweeping machine and other fi elds, and a relatively optimized scheme can be obtained from the aspect of time and energy consumption

Mammospheres from murine mammary stem cell-enriched basal cells: Clonal characteristics and repopulating potential

AbstractIdentification of murine mammary stem cells (MaSCs) has been attempted with various in vitro and in vivo assays. While, the in vivo repopulation assay remains as the most definitive assay for MaSC detection, it is expensive, time-consuming, and technically challenging. The in vitro mammosphere assay was considered unreliable because of major concerns about its clonal origin. In the current study, co-culture experiments with mammary cells from fluorescent protein transgenic mice and time-lapse video microscopy revealed that >90% mammospheres formed from sorted basal epithelial-enriched cells were of clonal origin in terms of stem cell. These basal-cell derived mammospheres were further distinguished morphologically in a 3-dimensional extracellular matrix culture and functionally in the in vivo repopulation assay. Transplant of single mammospheres or the resultant 3-dimensional solid structures into gland-free mammary fat pads yielded a 70% success rate of multilineage mammary gland reconstitution. Thus, this in vitro sphere formation and differentiation assay is a reliable alternative to the in vivo repopulation assay for the study of MaSCs

Effect of Biocontrol Agent Pseudomonas fluorescens 2P24 on Soil Fungal Community in Cucumber Rhizosphere Using T-RFLP and DGGE

Fungi and fungal community play important roles in the soil ecosystem, and the diversity of fungal community could act as natural antagonists of various plant pathogens. Biological control is a promising method to protect plants as chemical pesticides may cause environment pollution. Pseudomonas fluorescens 2P24 had strong inhibitory on Rastonia solanacearum, Fusarium oxysporum and Rhizoctonia solani, etc., and was isolated from the wheat rhizosphere take-all decline soils in Shandong province, China. However, its potential effect on soil fungal community was still unknown. In this study, the gfp-labeled P. fluorescens 2P24 was inoculated into cucumber rhizosphere, and the survival of 2P24 was monitored weekly. The amount decreased from 108 to 105 CFU/g dry soils. The effect of 2P24 on soil fungal community in cucumber rhizosphere was investigated using T-RFLP and DGGE. In T-RFLP analysis, principle component analysis showed that the soil fungal community was greatly influenced at first, digested with restriction enzyme Hinf I and Taq I. However, there was little difference as digested by different enzymes. DGGE results demonstrated that the soil fungal community was greatly shocked at the beginning, but it recovered slowly with the decline of P. fluorescens 2P24. Four weeks later, there was little difference between the treatment and control. Generally speaking, the effect of P. fluorescens 2P24 on soil fungal community in cucumber rhizosphere was just transient

Long-term Variations in Wintertime Arctic Frontal Positions and Their Mercury Anthropogenic Emission Impacts

Wintertime Arctic frontal positions are particularly important in determining the inclusion of mercury emissions from anthropogenic sources in the Arctic. Using the streamline confluence method, the 30-year average positions of wintertime (November-March) Arctic fronts exhibited similar spatial variability (39°N-67°N) over North America and Eurasia during 1988-2017 with considerable interannual variations up to 10° year-to-year shifts in latitude. The long-term variations in Arctic frontal positions partly superseded the impact of the decreasing North American anthropogenic mercury emissions by up to 30%, leading to no significantly detectable emission trend in the North American Arctic. The northward shifts in Arctic frontal positions in some winters in Eurasia have counteracted the impacts of anthropogenic emission changes by 5.3%. This is the first study to examine and quantify the impact of long-term variations in Arctic frontal positions on Arctic anthropogenic mercury emissions; it provides insight into the impact of climate change on Arctic anthropogenic emissions

DDOST is associated with tumor immunosuppressive microenvironment in cervical cancer

Abstract Evidence has revealed that DDOST plays an important role in cancer development and progression. However, there are no reports on functions of DDOST in cervical tumorigenesis. Hence, we investigated the relationship of DDOST with prognosis, mutation, promoter methylation, immune cell infiltration, and drug sensitivity using bioinformatics techniques. Our results demonstrated that DDOST was significantly upregulated in a variety of tumor types and correlated with poor prognosis, including cervical cancer. Cox regression analysis dissected that high DDOST expression was associated with poor survival in cervical cancer patients. Immune infiltration analysis defined that DDOST was negatively correlated with CD8 T cells and NK cells. Strikingly, the sensitivity to multiple drugs was negatively correlated with the expression of DDOST. Therefore, our findings uncovered that DDOST could play an essential role in the tumor microenvironment and tumor immune regulation in cervical cancer, which indicated that DDOST could be a useful biomarker for prognosis and a potential therapeutic target for cancer treatment