8 research outputs found

    Parasitological inquiry in dogs with guardians in the city of Porto Alegre, Rio Grande do Sul (2013-2019)

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    Cães são hospedeiros reservatórios de espécies de helmintos e protozoários e a coabitação com humanos desempenha um papel fundamental na transmissão zoonótica. O objetivo deste estudo é relatar a prevalência de helmintos e protozoários intestinais e estabelecer os principais endoparasitos em cães domiciliados da cidade de Porto Alegre, Rio Grande do Sul. Amostras fecais foram coletadas de 1400 cães durante os anos de 2013-2019 e analisadas no Laboratório de Helmintoses da Faculdade de Veterinária da Universidade Federal do Rio Grande do Sul. No total, 316 (22,6%) amostras foram positivas ao menos para um gênero parasitário, com taxas de 62,4%, 43,8%, 23,8%, 14,6%, 8,8%, 18,3% e 30,8%, respectivamente para os anos de 2013, 2014, 2015, 2016, 2017, 2018 e 2019. A taxa de positividade para cães monoinfectados foi de 86,4% (273/316) e de 13,6% (43/316) para poliparasitados, com destaque para Ancylostoma spp. Os resultados reforçam a necessidade de melhorar os programas de controle de parasitos intestinais para proteger cães e humanos e a necessidade de aumentar o conhecimento dos tutores de cães sobre o assunto.Dogs a reservoir hosts for helminth and protozoan species and cohabitation with humans plays a key role in zoonotic transmission. The aim of this study is to report the prevalence of intestinal helminths and protozoa and stablish the principal endoparasites in domestic dogs in the city of Porto Alegre, Rio Grande do Sul. Fecal samples were collected from 1400 dogs during the years 2013-2019 and analyzed at the Helminths Laboratory of the Faculty of Veterinary Medicine at the Federal University of Rio Grande do Sul. In total, 316 (22,6%) samples were positive for the least one parasite genus, with rates of 62,4%, 43,8%, 23,8%, 14,6%, 8,8%, 18,3% and 30,8%, respectively for the years 2013, 2014, 2015, 2016, 2017, 2018 and 2019. The positivity rate for monoinfected dogs was 86,4% (273/316) and 13,6% (43/316) for polyparasited dogs, especially Ancylostoma spp. The results reinforce the need to improve intestinal parasite control programs to protect dogs and humans and the need to increase the knowledge of dog tutors on the subject

    Rastreio de parasitos gastrointestinais potencialmente zoonóticos em populações caninas do estado de Sergipe, Brasil.

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    O seguinte trabalho expõe as atividades realizadas durante o Estágio Supervisionado Obrigatório (ESO) do discente Allan Costa Gomes, sendo o mesmo realizado no período de 12 de junho a 05 de outubro de 2023, com duração total de 656 horas integradas, no Laboratório de Patologia Clínica Veterinária-LPCV, sendo supervisionado durante todo o período de estágio pela Profa. Dra. Miriam Nogueira Teixeira. Além disso o trabalho expõe o Trabalho de Conclusão de Curso-TCC do discente, levando em consideração o aprimoramento do mesmo em todas as vertentes práticas da patologia clínica e ainda pensando-se em evidenciar os principais parasitos gastrointestinais zoonóticos presentes na região de Sergipe. Diante disso, o projeto de pesquisa é intitulado: Rastreio de parasitos gastrointestinais potencialmente zoonóticos em populações caninas do estado de Sergipe, Brasil, sob supervisão do Prof. Dr. Victor Fernando Santana Lima. Através do estágio, o discente teve total envolvimento com a prática laboratorial de rotina veterinária, proporcionando assim, o desenvolvimento na habilidade e compreensão acerca do entendimento, execução, realização e diagnóstico dos exames laboratoriais, sendo de grande importância para a manutenção da saúde e bem-estar dos animaisNossa Senhora da Glóri

    Ultrasensitive detection of toxocara canis excretory-secretory antigens by a nanobody electrochemical magnetosensor assay.

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    peer reviewedHuman Toxocariasis (HT) is a zoonotic disease caused by the migration of the larval stage of the roundworm Toxocara canis in the human host. Despite of being the most cosmopolitan helminthiasis worldwide, its diagnosis is elusive. Currently, the detection of specific immunoglobulins IgG against the Toxocara Excretory-Secretory Antigens (TES), combined with clinical and epidemiological criteria is the only strategy to diagnose HT. Cross-reactivity with other parasites and the inability to distinguish between past and active infections are the main limitations of this approach. Here, we present a sensitive and specific novel strategy to detect and quantify TES, aiming to identify active cases of HT. High specificity is achieved by making use of nanobodies (Nbs), recombinant single variable domain antibodies obtained from camelids, that due to their small molecular size (15kDa) can recognize hidden epitopes not accessible to conventional antibodies. High sensitivity is attained by the design of an electrochemical magnetosensor with an amperometric readout with all components of the assay mixed in one single step. Through this strategy, 10-fold higher sensitivity than a conventional sandwich ELISA was achieved. The assay reached a limit of detection of 2 and15 pg/ml in PBST20 0.05% or serum, spiked with TES, respectively. These limits of detection are sufficient to detect clinically relevant toxocaral infections. Furthermore, our nanobodies showed no cross-reactivity with antigens from Ascaris lumbricoides or Ascaris suum. This is to our knowledge, the most sensitive method to detect and quantify TES so far, and has great potential to significantly improve diagnosis of HT. Moreover, the characteristics of our electrochemical assay are promising for the development of point of care diagnostic systems using nanobodies as a versatile and innovative alternative to antibodies. The next step will be the validation of the assay in clinical and epidemiological contexts

    Zoonotic Soil-Transmitted Helminths in Free-Roaming Dogs, Kiribati

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    Soil-transmitted helminths are highly prevalent in the Asia-Pacific region. We report a 96.5% prevalence of zoonotic soil-transmitted helminths in dogs in Kiribati. We advocate for urgent implementation of treatment and prevention programs for these zoonotic pathogens, in line with the Kiribati-World Health Organization Cooperation Strategy 2018-2022

    Biological Invasions in South Africa

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    This open access volume presents a comprehensive account of all aspects of biological invasions in South Africa, where research has been conducted over more than three decades, and where bold initiatives have been implemented in attempts to control invasions and to reduce their ecological, economic and social effects. It covers a broad range of themes, including history, policy development and implementation, the status of invasions of animals and plants in terrestrial, marine and freshwater environments, the development of a robust ecological theory around biological invasions, the effectiveness of management interventions, and scenarios for the future. The South African situation stands out because of the remarkable diversity of the country, and the wide range of problems encountered in its varied ecosystems, which has resulted in a disproportionate investment into both research and management. The South African experience holds many lessons for other parts of the world, and this book should be of immense value to researchers, students, managers, and policy-makers who deal with biological invasions and ecosystem management and conservation in most other regions

    Biological Invasions in South Africa

    Get PDF
    This open access volume presents a comprehensive account of all aspects of biological invasions in South Africa, where research has been conducted over more than three decades, and where bold initiatives have been implemented in attempts to control invasions and to reduce their ecological, economic and social effects. It covers a broad range of themes, including history, policy development and implementation, the status of invasions of animals and plants in terrestrial, marine and freshwater environments, the development of a robust ecological theory around biological invasions, the effectiveness of management interventions, and scenarios for the future. The South African situation stands out because of the remarkable diversity of the country, and the wide range of problems encountered in its varied ecosystems, which has resulted in a disproportionate investment into both research and management. The South African experience holds many lessons for other parts of the world, and this book should be of immense value to researchers, students, managers, and policy-makers who deal with biological invasions and ecosystem management and conservation in most other regions

    Biological Invasions in South Africa

    Get PDF
    This open access volume presents a comprehensive account of all aspects of biological invasions in South Africa, where research has been conducted over more than three decades, and where bold initiatives have been implemented in attempts to control invasions and to reduce their ecological, economic and social effects. It covers a broad range of themes, including history, policy development and implementation, the status of invasions of animals and plants in terrestrial, marine and freshwater environments, the development of a robust ecological theory around biological invasions, the effectiveness of management interventions, and scenarios for the future. The South African situation stands out because of the remarkable diversity of the country, and the wide range of problems encountered in its varied ecosystems, which has resulted in a disproportionate investment into both research and management. The South African experience holds many lessons for other parts of the world, and this book should be of immense value to researchers, students, managers, and policy-makers who deal with biological invasions and ecosystem management and conservation in most other regions
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