A study of the root surface phosphatase activities of three species of higher plants: Juncus effusus, Phragnutes australis, and Typha latifolia

The aim of this project was to investigate a possible link between environmental phosphorus status and the root surface phosphatase activities of three species of emergent macrophytes, with a view to assessing their potential for use as "biondicators". Analyses of water phosphorus concentrations and rates of surface phosphatase activities of Juncus effusus, Phragmites australis and Typha latifolia were carried out on samples from Bakethin Reservoir, Northumberland and Durham University Botanic Garden. Differences were found in water phosphorus concentrations at the sites. Water from Bakethin Reservoir was found to have lower levels of P than water from the Botanic Garden. Interspecific differences at p = 0.0002 were discovered in rates of phosphatase activity between Juncus, Phragmites and Typha. Juncus and Typha were found to exhibit significantly lower rates of phosphatase activity at the Botanic Garden than at Bakethin reservoir (p = 0.026 for Juncus, p = 0.037 for Typha). High rates of phosphatase activity in Juncus and Typha at Bakethin Reservoir corresponded with low concenfrations of environmental phosphorus, so it is possible that root surface phosphatases of both species are inducible in conditions of P- limitation. Juncus and Typha may therefore have the potential for use as bioindicators of environmental phosphorus status. Several practical problems were encountered, and may be of general significance. Rates of phosphatase activity declined rapidly in 100 μM pNPP assays. As a consequence, assays were terminated after 10 min. It was also observed that roots removed after assays had been terminated often showed a yellow coloration, presumably due to the retention of pNP. The accuracy of the pNPP assay relies upon all the pNP produced by the hydrolysis of pNPP being released into solution. The apparent retention of pNP by roots therefore brings the use of the pNPP assay, as a method for determining rates of phosphatase activity in eukaryotes, under scrutiny. One preliminary experiment carried out showed that more pNP was retained by roots under conditions of low pH

A Typha Angustifolia-like MoS2/carbon nanofiber composite for high performance Li-S batteries

A Typha Angustifolia-like MoS2/carbon nanofiber composite as both a chemically trapping agent and redox conversion catalyst for lithium polysulfides has been successfully synthesized via a simple hydrothermal method. Cycling performance and coulombic efficiency have been improved significantly by applying the Typha Angustifolia-like MoS2/carbon nanofiber as the interlayer of a pure sulfur cathode, resulting in a capacity degradation of only 0.09% per cycle and a coulombic efficiency which can reach as high as 99%

The Growth and Survival of Early Instars of \u3ci\u3eBellura Obliqua\u3c/i\u3e (Lepidoptera: Noctuidae) on \u3ci\u3eTypha Latifolia\u3c/i\u3e and \u3ci\u3eTypha Angustifolia\u3c/i\u3e

Larvae of the noctuid moth Bellura obliqua are frequently encountered on Typha latifolia, but less commonly on Typha angustifolia. Experiments were conducted to compare the growth and survivorship of early B. obliqua instars on the two species of cattail. In short-term growth chamber experiments there were no significant differences in the survivorship, relative growth rate (RGR), relative consumption rate (RCR), or the efficiency of conversion of ingested food (ECI) between first-instar larvae reared on leaves of the two species. Third-instar larvae fed stems, however, had a greater RGR and higher ECI when reared on T. lalifolia. Differences in growth are apparently not related to differences in hostplant nitrogen or acid-detergent fiber content. In a long term greenhouse experiment, using transplanted cattails, larvae reared on T. latifolia grew somewhat larger and had a significantly higher survival rate than those reared on T. angustifolia. Host plant structure is postulated to influence larval survivorship. Typha is under consideration for use as a bio-energy crop and planting T. angustifolia may help to reduce infestations in cultivated stands

An assessment of the cost effectiveness of vegetation harvesting as a means of removing nutrient and metals from ponds

This paper reports on an investigation to quantify the mass of pollutants removed from a stormwater retention pond by routine vegetation harvesting. The amount of plants can increase the costs of ponds, and the increased costs of plant maintenance may not be justified by enhanced pollutant removal. This study provides some of the basic information, previously lacking, which is needed to come to such decisions. The study facility was La Costa pond, a retention pond in California used to treat highway runoff. Water quality monitoring data indicate that the pond removed 43 percent of the total nitrogen entering the facility, with 5 to 7 percent directly attributable to harvesting the vegetation – in this case cattails (Typha). The data also indicate that 48 percent of the total annual phosphorus was removed from the runoff, with the harvested vegetation responsible for between 3 and 8 percent. Metal uptake by the vegetation was substantially less than nutrients. Total removal of copper, lead and zinc by the pond varied between 57 and 93 percent, with the harvested vegetation accounting for less than 2 percent of removal. Issues addressed in the paper include the cost implications of harvesting and ways of improving vegetative pollutant removal

Araneae and Opiliones From \u3ci\u3eTypha\u3c/i\u3e Spp. And \u3ci\u3ePhragmites Australis\u3c/i\u3e Stands of Green Bay, Lake Michigan, and an Exotic Spider Species Newly Reported From the U.S. Great Lakes Region

Invertebrates were sampled using pan traps in three paired sets of Typha spp. (cattail) and Phragmites australis (giant reed grass) habitats in Lake Michigan’s Green Bay in June and September 2002. The collection included 34 harvestmen belonging to one species (found at all three sites), and 180 spiders belonging to 25 species in eight families. The two habitats yielded similar numbers of spider taxa, and 16 species were restricted to one of the two habitats. Between 10 and 15 species were found at each site, and only five spider species were collected at all three sites. Four species appear to be new records for the state of Wisconsin, including the linyphiid spiders Hypomma marxii (Keyserling) and Sitalcus ruralis Bishop & Crosby, and the salticid Synageles noxiosus (Hentz). Of particular interest is the first report from the U.S. Great Lakes region of the clubionid spider Clubiona pallidula (Clerck), a species introduced from Eurasia

The botany and proximate analyses of some edible species of the New Zealand flora : a thesis presented in partial fulfilment of the requirement for the degree of Master of Science in Plant Biology at Massey University

1. The edible organs of some New Zealand plant species have been assessed at light microscope level for their botanical basis, and for their nutritional basis by proximate analysis and Plasma Emission Spectrometry. 2. The species investigated, listed by Colenso (1880) as the most valued plant foods of the pre-European Maori, were: Pteridium esculentum (rhizome), Corynocarpus laevigatus (kernel), Elaeocarpus dentatus, (pericarp), Sonchus asper (herb), Calystegia sepium (rhizome), Cyathea medullaris (frond stipe), Cordyline australis (leaf bases, root), Rhopalostylis sapida (apical shoot), Typha orientalis (pollen and rhizome), Beilschmiedia tawa (kernel), Marattia salicina (rhizomal scale), Porphyra columbina (frond), Auricularia polytricha (basidiocarp), Arthropodium cirratum (rhizome), Bolboschoenus fluviatilis (rhizomal tuber), Gastrodia cunninghamii (rhizome) and Asplenium bulbiferum (immature frond). 3. Specimens were collected at the appropriate traditional seasons (except for Gastrodia cunninghamii) and samples prepared by freeze-drying and milling. Samples were also prepared of the cooked organs of Corynocarpus laevigatus, Elaeocarpus dentatus, Sonchus asper, Cyathea medullaris, Beilschmiedia tawa and Porphyra columbina. 4. Analytical determinations were made for lipid, by extraction with di-ethyl ether; nitrogen, by micro-Kjeldahl method and colorimetric measurement of ammonia using indophenol; protein, by Bradford procedure using Coomassie Brilliant Blue and colorimetry; dietary fibre, by Englyst procedure using enzymatic digestion and colorimetry; soluble sugar, by acid hydrolysis and colorimetry; and starch, by enzymatic digestion and colorimetry. 5. Botanical investigations were made following histological procedures and microtechnique using paraffin wax embedding and staining with safranin and fast green; and by differential staining of hand-cut sections using Sudan Blue, iodine and Coomassie Brilliant Blue. 6. Analytical determinations were made for 23 trace, minor and major constituent elements, using inductively-coupled argon plasmas in a simultaneous emission spectrometer. 7. Proximate analyses showed high levels of lipid in Corynocarpus laevigatus, Cyathea medullaris, and Sonchus asper, of protein in Corynocarpus laevigatus, Sonchus asper, Rhopalostylis sapida, Typha orientalis (pollen) and Asplenium bulbiferum; of dietary fibre in Auricularia polytricha, Beilschmiedia tawa, Marattia salicina (root) and Porphyra columbina (uncooked); of soluble sugar in Cyathea medullaris, Cordyline australis (leaf bases and root), Typha orientalis (rhizomes and pollen) and Pteridium esculentum; and of starch in Corynocarpus laevigatus, Elaeocarpus dentatus, Marattia salicina, Calystegia sepium and Gastrodia cunninghamii. 8. High levels of essential minerals and trace elements were measured in many samples, and some excess levels of toxic metals were recorded. 9. The nutritional and ethnobotanical aspects of a pre-European Maori diet were related to the analytical and botanical findings of the investigation

Stomatal control of leaf fluxes of carbonyl sulfide and CO₂ in a <i>Typha</i> freshwater marsh

Carbonyl sulfide (COS) is an emerging tracer to constrain land photosynthesis at canopy to global scales, because leaf COS and CO2 uptake processes are linked through stomatal diffusion. The COS tracer approach requires knowledge of the concentration normalized ratio of COS uptake to photosynthesis, commonly known as the leaf relative uptake (LRU). LRU is known to increase under low light, but the environmental controls over LRU variability in the field are poorly understood due to scant leaf scale observations.Here we present the first direct observations of LRU responses to environmental variables in the field. We measured leaf COS and CO2 fluxes at a freshwater marsh in summer 2013. Daytime leaf COS and CO2 uptake showed similar peaks in the mid-morning and late afternoon separated by a prolonged midday depression, highlighting the common stomatal control on diffusion. At night, in contrast to CO2, COS uptake continued, indicating partially open stomata. LRU ratios showed a clear relationship with photosynthetically active radiation (PAR), converging to 1.0 at high PAR, while increasing sharply at low PAR. Daytime integrated LRU (calculated from daytime mean COS and CO2 uptake) ranged from 1 to 1.5, with a mean of 1.2 across the campaign, significantly lower than the previously reported laboratory mean value (∼ 1.6). Our results indicate two major determinants of LRU – light and vapor deficit. Light is the primary driver of LRU because CO2 assimilation capacity increases with light, while COS consumption capacity does not. Superimposed upon the light response is a secondary effect that high vapor deficit further reduces LRU, causing LRU minima to occur in the afternoon, not at noon. The partial stomatal closure induced by high vapor deficit suppresses COS uptake more strongly than CO2 uptake because stomatal resistance is a more dominant component in the total resistance of COS. Using stomatal conductance estimates, we show that LRU variability can be explained in terms of different patterns of stomatal vs. internal limitations on COS and CO2 uptake. Our findings illustrate the stomata-driven coupling of COS and CO2 uptake during the most photosynthetically active period in the field and provide an in situ characterization of LRU – a key parameter required for the use of COS as a photosynthetic tracer

Untersuchungen zur Renaturierung von Feuchtgebieten im Tijuana Ästuar unter besonderer Berücksichtigung von brackwasserhaltigen und salinen Teichen

This study represents the first comprehensive biological and hydrochemical investigation of small coastal ponds in the saltmarsh dominated Tijuana Estuary, southern California (U.S.). Special attention is given to the brackish water biotopes. Different salinities and considerable fluctuations in water level characterized these shallow ponds and restrict the biological settlement. Fluctuations of salinities ranged from brackish water to hyperhaline water conditions. Due to different salinity levels, the ponds vary in hydrochemistry, macroinvertebrate species composition and plant communities. The macroinvertebrate community of the brackish waters were dominated by Gastropoda, Odonata, and Coleoptera containing a mixture of freshwater/brackish water species and marine macroinvertebrates. Typical plants of the brackish habitat were Typha domingensis (Southern cattail), and Scirpus californicus (California bulrush) associated with Juncus acutus (Siny rush). These brackish habitats with a wide range of salinity fluctuations are sparsely colonized but represent a niche for typical highly adaptable species. Especially, it is a biotope for species with a wide range of salt tolerance. Therefore, endangered species occurred besides introduced or invasive species in the ponds of the Tijuana Estuary. This fact has to be taken into account in case of wetland restoration. Due to the freshwater influence, the restoration of brackish habitats focuses on the problem of invasive species.Die vorliegende Arbeit befasst sich mit der Situation des Tijuana-River-Ästuars im Südwesten Kaliforniens und untersucht insbesondere Flachgewässer mit Brackwassercharakter. Verschiedene Salzgehalte und erhebliche Schwankungen der Wasserstände sind für diese Gewässer charakteristisch und limitieren die biologische Besiedelung. Die Unterschiede in der Salinität reichen dabei von Brackwasserbedingungen bis hin zur Hypersalinität. Aufgrund der verschiedenen Salzgehalte variieren die Gewässer in der hydrochemischen Zusammensetzung, der Makroinvertebratenzusammensetzung und der Pflanzengesellschaften. Die Gruppe der Makroinvertebraten in den Brackwasserbiotopen wird von Schnecken, Libellen und Käfern dominiert, wobei Süß- und Brackwasserarten zusammen mit marinen Invertebraten auftreten. Typische Pflanzen der Brackwasserlebensräume sind Typha domingensis und Scirpus californicus, verbunden mit Juncus acutus-Pflanzen. Solche Brackwasserbiotope mit ihren stark schwankenden Salzgehalten sind zwar artenarm, aber sie stellen zugleich Nischen für Organismen mit speziellem hohem Adaptationsvermögen insbesondere hinsichtlich der Salztoleranz dar. So wurden bei den Untersuchungen gefährdete, aber auch invasive Arten gefunden. Diese Besonderheit ist bei allen Sanierungs- und Renaturierungsaktivitäten zu berücksichtigen

Ecological modelling of a wetland for phytoremediating Cu, Zn and Mn in a gold–copper mine site using Typha domingensis (Poales: Typhaceae) near Orange, NSW, Australia

Abstract: An artificial wetland was computationally modelled using STELLA®, a graphical programming tool&nbsp;for an Au–Cu mine site in Central-west NSW, the aim of which was to offer a predictive analysis of a proposed&nbsp;wetland for Cu, Zn and Mn removal using Typha domingensis as the agent. The model considers the important&nbsp;factors that impact phytoremediation of Cu, Zn and Mn. Simulations were performed to optimise the area of&nbsp;the wetland; concentration of Cu, Zn and Mn released from mine (AMD); and flow rates of water for maximum&nbsp;absorption of the metals. A scenario analysis indicates that at AMD = 0.75mg/L for Cu, Zn and Mn, 12.5, 8.6,&nbsp;and 357.9 kg of Cu, Zn and Mn, respectively, will be assimilated by the wetland in 35 years, which would be equivalent to 61 mg of Cu/kg, 70 mg of Zn/kg and 2,886 mg of Mn/kg of T. domingensis, respectively. However, should Cu, Zn and Mn in AMD increase to 3 mg/L, then 18.6 kg of Cu and 11.8 kg of Zn, respectively, will be&nbsp;assimilated in 35 years, whereas no substantial increase in absorption for Mn would occur. This indicates that&nbsp;91 mg of Cu, 96 mg of Zn and 2917 mg of Mn will be assimilated for every kg of T. domingensis in the wetland.&nbsp;The best option for Cu storage would be to construct a wetland of 50,000 m2 area (AMD = 0.367 mg/L of Cu),&nbsp;which would capture 14.1 kg of Cu in 43 years, eventually releasing only 3.9 kg of Cu downstream. Simulations&nbsp;performed for a WA of 30,000 m2 indicate that for AMD = 0.367 mg/L of Zn, the wetland captures 6.2 kg, releasing&nbsp;only 3.5 kg downstream after 43 years; the concentration of Zn in the leachate would be 10.2 kg, making&nbsp;this the most efficient wetland amongst the options considered for phytoremediating Zn. This work will help&nbsp;mine managers and environmental researchers in developing an effective environmental management plan by&nbsp;focusing on phytoremediation, with a view at extracting Cu, Zn and Mn from the contaminated sites

Effects of Typha x glauca on methane emissions in freshwater ecosystems: implications of invasive species effects on global climate change.

Undergraduate Research Exper.Across the globe, freshwater ecosystems play vital roles in global nutrient and emission cycles and provide key ecosystem services. Great Lakes wetlands have increasingly been dominated by an invasive cattail hybrid? Typha x glauca. Cheboygan Marsh, a Great Lake marsh formed by Lucustrine deposits, was invaded by Typha in the 1950’s. Resent surveys indicate Typha now dominates two-thirds of the ~150 ha marsh. Typha has the propensity to alter freshwater ecosystems by forming dense stands of live and dead biomass. As Typha biomass accumulates and decomposes in the soil it can act as a fuel for methanogenesis when coupled with anaerobic conditions often found in these ecosystems. As tipping points in global climate change are quickly being approached research on both anthropogenic and natural emissions are warranted. In this study we used both a field and mesocosm experiment to test for the effect Typha invasion is having through its leaf litter on methane emissions. We found soil organic carbon to be in much higher concentrations in Typha zones than Native vegetation zones dominated by sedges and rushes in Cheboygan Marsh. In the mescocom array we also found a positive correlation between higher soil organic carbon and increased methane flux rates. These finding may suggest that the possibility exists for Typha invasion to play a significant role in future climate change.http://deepblue.lib.umich.edu/bitstream/2027.42/89585/1/Castillo_Buck_2011_REU.pd