Synthesis and Chemical Nuclease Activities of Copper (II) and Ni (II) Complexes of 4-Chloro-2-(pyridine-3-Yliminomethyl) Phenol Schiff Bases

This communication describes a procedure to synthesize a novel Schiff base ligand4-chloro-2-(pyridine-3-yliminomethyl) phenol and its transition metal complexes of Cu(II) and Ni(II)and its characterization by physiochemical techniques such as elemental, FTIR,UVVisible, 1 H-NMR, Mass spectra and cyclic voltammetry. Further, the ligand was structurally characterized by X-ray crystallography and is found to be planar suitable for DNA studies. The complexes were screened for their in-vitro antimicrobial activity using various strains of gram positive and gram negative bacteria- Bacillus mycoides, Bacillus subtilis, Escherichia coli, Micrococcus luteus, Proteus mirabilis, Pseudomonas aeruginosa and Yersinia enterocolitica. The Schiff base ligand showed higher antibacterial activity than the ligand coordinated to either of the metal ion copper or nickel. The DNA cleavage studies of the complexes have been investigated and both the complexes showed extensive cleavage of DNA. However, Cu(II) complexes showed better cleavage activity compared to Ni(II) complex

Cu(II) and Fe(III) complexes of sulphadoxine mixed with pyramethamine: Synthesis, characterization, antimicrobial and toxicology study

Two new mixed ligands metal complexes of sulphadoxine and pyramethamine were prepared by using CuCl2.6H2O and FeCl3.6H2O. The complexes were characterized by elemental analysis, melting point determination, molar conductivity, metal content analysis (AAS), IR, magnetic susceptibility measurements and UV-Visible spectroscopy. Based on the analytical and spectroscopic data, the complexes were proposed to have the formulae [M1L1L2(Cl)2] and [M2L1L2(Cl)3] (where M1 = Cu(II), M2 = Fe(III)), L1 = sulphadoxine, L2 = pyramethamine). The spectroscopic data proposed L1 to be a monodentate ligand and coordinated through N atom of the NH2 group in both complexes. Also, L2 was proposed to be tridentate ligand and coordinated through N atom of the NH2 groups and through N atom of imine group. However, [M1L1L2(Cl)2] and [M2L1L2(Cl)3] were proposed to possess distorted octahedral geometry. Conductivity measurement values supported the non-electrolytic nature of the complexes. The complexes have been tested in vitro against a number of pathogenic bacteria [g(+) Escherichia coli, g(+) Proteus species, g(+) Pseudomonas aeruginosa and g(+) Salmonella typhi] by using disc diffusion method. Obtained results indicated that the metal complexes exhibited better antibacterial activities as compared to the ligands. Toxicology tests against some tissues of albino rat (Rattus novergicuss) revealed toxicity of the complexes in the kidney as compared to the parent drugs. [M1L1L2(Cl)2] was found to be toxic to the sera, livers and kidneys of the rats used, while [M2L1L2(Cl)3] was found to be non-toxic to the sera, livers and kidneys of the rats as their alkaline phosphatase (ALP) values showed non-significant difference to the control value

“In Vitro Antimicrobial Activity and DNA Cleavage Studies: Synthesis and Characterization of Novel M(II) Complexes with O, N, O donor Schiff Base Ligand Derived from Phenylpropanehydrazide”

Three new ternary complexes of general formulation [M(L)n] (1–3), where L=N`-(5-chloro-2-hydroxybenzylidine)-3- phenylpropanehydrazide; n=2;M= Cu, Ni, Zn, complexes are synthesized, characterized by various physicochemical and UV-Vis, FTIR, 1H NMR and ESI-MS spectroscopic methods. The Cyclic voltammetry show a quasi-reversible cyclic voltammetric response due to one electron Cu(II)/Cu(I) reduction near 100 mV (versus SCE) in DMF–0.1 M KCl. All the compounds were screened for their in-vitro antibacterial activity against Gram positive and Gram negative bacterial strains. Among them, Cu complex showed good activity against all microbes. The copper complex shows moderate chemical nuclease activity in the presence of MPA as a reducing agen

HISTOLOGICAL STUDIES OF BREWERY SPENT GRAINS IN DIETARY PROTEIN FORMULATION IN DONRYU RATS

The increasing production of large tonnage of products in brewing industries continually generates lots of solid waste which includes spent grains, surplus yeast, malt sprout and cullet. The disposal of spent grains is often a problem and poses major health and environmental challenges, thereby making it imminently necessary to explore alternatives for its management. This paper focuses on investigating the effects of Brewery Spent Grain formulated diet on haematological, biochemical, histological and growth performance of Donryu rats. The rats were allocated into six dietary treatment groups and fed on a short-term study with diet containing graded levels of spent grains from 0, 3, 6, 9, 12 and 100% weight/weight. The outcome demonstrated that formulated diet had a positive effect on the growth performance of the rats up to levels of 6% inclusions, while the haematological and biochemical evaluation revealed that threshold limit should not exceed 9% of the grain. However, the histological study on the liver indicated a limit of 3% inclusion in feed without serious adverse effect. Thus invariably showing that blend between ranges 1-3% is appropriate for the utilization of the waste in human food without adverse effect on the liver organ. The economic advantage accruing from this waste conversion process not only solves problem of waste disposal but also handle issues of malnutrition in feeding ration

In vitro antimicrobial activity and DNA cleavage studies: synthesis and characterization of novel M(II) complexes with tridentate [ONO] donor Schiff base ligand derived from phenylpropane hydrazide

Three new ternary complexes of general formulation [M(L)​n] (1-​3)​, where L = N'-​(5-​chloro-​2-​hydroxybenzylidine)​-​3-​phenylpropane hydrazide; n = 2; M = Cu, Ni, Zn, complexes are synthesized, characterized by various physicochem. and UV-​Vis, FT-​IR, 1H NMR and ESI-​MS spectroscopic methods. The Cyclic voltammetry show a quasi-​reversible cyclic voltammetric response due to one electron Cu(II)​/Cu(I) redn. near 100 mV (vs. SCE) in DMF-​0.1 M KCl. All the compds. were screened for their in-​vitro antibacterial activity against Gram pos. and Gram neg. bacterial strains. Among them, Cu complex showed good activity against all microbes. The copper complex shows moderate chem. nuclease activity in the presence of MPA as a reducing agent

Dangling and hydrolyzed ligand arms in [Mn3] and [Mn6] coordination assemblies: synthesis, characterization, and functional activity

Two flexible, branched, and sterically constrained di- and tripodal side arms around a phenol backbone were utilized in ligands H3L1 and H5L2 to isolate {Mn6} and {Mn3} coordination aggregates. 2,6-Bis{(1-hydroxy-2-methylpropan-2-ylimino)methyl}-4-methylphenol (H3L1) gave trinuclear complex [Mn3(μ-H2L1)2(μ1,3-O2CCH3)4(CH3OH)2](ClO4)2·4CH3OH (1), whereas 2,6-bis[{1-hydroxy-2-(hydroxymethyl)butan-2-ylimino}methyl]-4-methylphenol (H5L2) provided hexanuclear complex [Mn6(μ4-H2L2)2(μ-HL3)2(μ3-OH)2(μ1,3-O2CC2H5)4](ClO4)2·2H2O (2). Binding of acetates and coordination of {H2L1}− provided a linear MnIIIMnIIMnIII arrangement in 1. A MnIII6 fused diadamantane-type assembly was obtained in 2 from propionate bridges, coordination of {H2L2}3–, and in situ generated {HL3}2–. The magnetic characterization of 1 and 2 revealed the properties dominated by intramolecular anti-ferromagnetic exchange interactions, and this was confirmed using density functional theory calculations. Complex 1 exhibited field-induced slow magnetic relaxation at 2 K due to the axial anisotropy of MnIII centers. Both the complexes show effective solvent-dependent catechol oxidation toward 3,5-di-tert-butylcatechol in air. The catechol oxidation abilities are comparable from two complexes of different nuclearity and structure