Antioxidant and antihemolytic activities of methanol extract of Hyssopus angustifolius

This study was designed to evaluate antioxidant and antihemolytic activities of Hyssopus angustifolius flower, stem and leaf methanol extracts by employing various in vitro assays. The leaf extract showed the best activity in DPPH (63.2 ± 2.3 μg mL-1) and H2O2  (55.6 ± 2.6 μg mL-1) models compared to the other extracts. However, flower extract exhibited the highest Fe2+ chelating activity (131.4 ± 4.4 μg mL-1). The extracts exhibited good antioxidant activity in linoleic acid peroxidation and reducing power assays, but were not comparable to vitamin C. The stem (23.58 ± 0.7 μg mL-1) and leaf (26.21 ± 1 μg mL-1) extracts showed highest level of antihemolytic activity than the flower extract

Effects of mulberry leaf extracts on activity and mRNA expression of five cytochrome P450 enzymes in rat

Adverse side effects of drug-drug interactions induced by human cytochrome P450 (CYP450)inhibition is an important consideration in drug discovery. Mulberry leaves are of broad popular use for food or remedy purposes, which is believed to contain substances that are beneficial for preventing and alleviating diabetes. However, there is a paucity of information about the effect of mulberry leaves on rat CYP450 enzymes activities and the mRNA expression levels in vivo. The present study aimed to investigate the effect of mulberry leaves on activities of rat CYP450 enzymes (CYP3A4, CYP2C8, CYP2C19, CYP2D6, and CYP1A2) through both probe-drug cocktail approach and real-time polymerase chain reaction (RT-qPCR). The pharmacokinetic results indicated that the aqueous extract of mulberry leaves (AML) exhibited induction effects on CYP3A4 activities, and AML exhibited inhibitory effects on CYP1A2, CYP2D6, and CYP2C8, while no obvious effect was observed on CYP2C19 activity. Additionally, the ethanol extract of mulberry leaves (EML) could induce the activities of CYP3A4. In addition, EML exhibited inhibitory effects on CYP1A2, CYP2D6, and CYP2C19, while no significant change in CYP2C8 activity was observed. Accordingly, the level of mRNA expression of five CYP enzymes were consistent with the result of pharmacokinetic. The results of our study may form a practical strategy for assessing CYP-mediated HDI

Efficacy of Some Native Botanical Extracts on the Repellency Property Against the Pink Mealy Bug, Maconellicoccus hirsutus (Green) in Mulberry Crop

Mulberry, Morus alba, (L.) leaves are the predominant food source for Silkworm, Bombyx mori rearing.  Pink mealy bug infests the mulberry plants and cause Tukra diseases that leads to qualitative loss of leaves.  Hence a study was carried out to evaluate the efficacy of various indigenous native botanical extracts for their repellency property against pink mealy bug Maconellicoccus hirsutus (Green) at the Tamil Nadu Agricultural University, Coimbatore.  The native botanicals such as Andrographis leaf extract, Leucas leaf extract, Neem seed kernel extract, vitex leaf extract, fish oil rosin soap, ocimum leaf extract and lawsonia leaf extract at different dose levels viz., 1, 2, 4, 8 and 10 per cent respectively. After 48 Hor (Hour of release) the highest repellency was recorded in case of Andrographis leaf extract (99.0%) followed by Leucas leaf extract and NSKE (99.0%). Vitex leaf extract and FORS showed on par results among various treatments. The ocimum leaf extract (90.1%) also recorded a moderate repellent effect and the least repellency was recorded in case of Lawsonia leaf extract (81.3%). Similar trend was recorded during 24 hour of release also. As the dose increases the repellent effect also increased irrespective of the native botanical extracts against mealy bugs

The Effectivity of Fermented Mulberry Leaves with Rumen Liquor as Broiler Feed on Final Body Weight, Dry Matter and Crude Fiber Digestibility, and Metabolic Energy

. Objective of this research was to know the effect of fermented mulberry leaves by rumen liquorin boiler's diet measured from final body weight, dry matter digestibility, crude fiber digestibility, and metabolic energy. One hundred broiler strain CP 707, broiler concentrate, mulberry leaves and other feed stuffs were administered in Completely Randomized Design with 5 treatments, namely T0 (control), T1 (10% mulberry leaves), T2 (10% fermented mulberry leaves), T3 (20% mulberry leaves) and T4 (20% fermented mulberry leaves) with 4 replications. Result revealed that the increasing use of mulberry leaves had significantly lowered final body weight, dry matter and fiber digestibility, metabolic energy. Fermentation treatment at 10% level could increase dry matter digestibility, crude fiber and metabolic energy than those of unfermented. This study concluded that the increasing mulberry leaves in broiler feed could reduce feed digestibility, and fermentation by rumen liquor could optimize the use of mulberry leaves as broilers feed

Synergism of virgin coconut oil and mulberry leaves extract as agent in free alcohol hand sanitizer / Norhazlin Jusoh ...[et al.]

Hand hygiene is foremost techniques in preventing the spread of any health care-associated infections and keeping the health cost under control. Hand sanitizer is the key for hand hygiene when limited resources and water access. The problem is alcohol-based hand sanitizer can cause health issues such as drying skin and alcoholic poisoning. Therefore, alcohol-free hand sanitizer becomes the next approach. Investigations on hand sanitizer without alcohol for their effectiveness on microorganism‟s infection are still scarce. Hence, the main objective of this study is to investigate the effectiveness of alcohol-free hand sanitizer on microbial hands carriage. The main focus is on inhibiting the growth of pure cultured bacteria from hands which were Streptococcus sp. and Staphylococcus sp. Production of alcohol-free hand sanitizer using natural products (virgin coconut oil and mulberry leaves extract) as antibacterial agents as an alternative to the current hand sanitizers in market. In the study, the antibacterial activity of alcohol-free hand sanitizer was compared with clinical hand sanitizer, commercial hand sanitizer and sterile water using disc diffusion method. Undoubtedly, clinical hand sanitizer showed the highest inhibition zone with 21 mm for both types of bacteria. On the other hand, alcohol-free hand sanitizer and commercial hand sanitizer surprisingly obtained almost similar size of inhibition zone with 14-14.5 mm. As conclusion, the alcohol-free hand sanitizer was effective in resisting the bacterial growth on hands and as promising option particularly in health impacts after use. The tested product can be introduced to selected market segments for example local pharmacies, clinics and hospital

桑抹茶の利用法に関する研究 〜ココアとの組み合わせによる抗酸化活性の変化について〜

In order to develop a new utilization method of mulberry leaf powder (MLP), this study tried to combine MLP with cocoa drink and measured antioxidant activity (AA) of combined sample solution expecting a synergistic effect. Contrary to expectation, AA of combined solutions was decreased with increasing in combined quantities of MLP by comparing with AA of each sample solution of MLP and cocoa drink. Reason of this result and effects of combination of MLP with cocoa drink on other functionalities are not yet known, so we cannot yet conclude whether this combination was not good

Potential Benefits of Plant-Derived Products on Broiler Meat Characteristics - A Short Review

Herbal supplements have been researched for decades as a safe alternative to synthetic antioxidants. These natural supplements may help broilers enhance their meat quality, growth, and physiological circumstances. The phenolic compounds in herbal products can serve as antioxidants, thereby responsible for lessening the negative effect of stress before slaughter. One of the most critical factors impacting broiler meats' physical, chemical, and oxidative stability is stress before slaughter. Reducing stress levels during rearing may be anticipated to enhance broiler meat qualities. Some studies have found that herbal supplementation improves meat qualities, whereas others have no impact. The efficacy of herbal supplementation on meat characteristics may be determined by the nature and levels of herbal supplementation and the condition of the animals throughout the rearing. The use of plant-derived products in broiler production and their impact on carcass characteristics, physical and organoleptic qualities, chemical compositions, and oxidative stability of broiler meats are covered in this review.

Evaluation of Antimicrobial Properties of Morus alba Grown on Silkworm Litter

Antibiotics provide the main basis for treating infectious diseases. Hence, there is an increase in the investigations on plants as a source of human disease management. Morus alba belonging to family Moraceae has been reported to possess several medicinal properties. In the present study the antimicrobial properties of mulberry plant grown in different conditions have been analysed. The main aim of the study was to compare the differences in the antimicrobial properties of mulberry grown on different organic manures such as farmyard manure and silkworm litter. The antimicrobial properties of aqueous, ethanolic and methanolic leaf extracts were tested against pathogenic bacteria such as E.coli and Pseudomonas and fungi such as Aspergillus niger and Penicillium notatum . Significant zone of inhibition were obtained for both bacterial pathogens when treated with extracts of mulberry grown on silkworm litter. Whereas with respect to fungi, FYM treated plant leaf extracts showed significant antifungal properties