Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil

Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities-Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever. © 2019 by the authors

Untargeted Alternative Routes of Arbovirus Transmission

Arboviruses have become global threats. Common to Dengue, Zika, yellow fever, chikungunya, and Mayaro viruses is their ability to be transmitted by mosquitoes. Several strategies based on transgenics or microbiology are currently being field-tested. While this approach seems hopeful, the research community needs to focus on potential backlash from these technologies to prevent failure. The aim of the Special Issue is to cover different transmission routes that are untargeted by the newly developed strategies to foresee limitations. Here, Fontenille & Powell gave their insights on how a mosquito species becomes a global vector, Yen & Failloux presented the limitations of Wolbachia-based population replacement, Pereira-dos-Santos et al. reviewed the evidence that Aedes albopictus is an important vector, and Diagne et al. gathered information about the latest emerging arbovirus: Mayaro. Manuel et al. demonstrated that in certain conditions mosquitoes efficiently transmit Zika viruses and Rozo-Lopez et al. showed that midges vertically transmit stomatitis virus, highlighting the epidemiological significance of vertical transmission. Vector competence for secondary vectors was improved by Kosoltanapiwat et al. during entomological surveillance and by Fernandes et al. when evaluating different vector species competence for Zika viruses. Morales-Vargas et al. and Calvez et al. improved our understanding of DENV2 and DENV4 epidemiology

The emergence of arboviruses changes the profile of viral meningitis in Salvador, Bahia: A case series

Background: Recently, different arboviruses became endemic in Brazil mostlycausing acute febrile illnesses, however, neurological manifestations have alsobeen reported. This study aimed to investigate which viruses were involved inthe meningitis etiology and the contribution of the circulating arboviruses inSalvador, Bahia, Brazil.Methods: From June 2014 to February 2016, 170 patients with suspected viralmeningitis were identified in Couto Maia Hospital, Salvador-BA, Brazil. TheirCSF samples were investigated for possible viral etiology by reversetranscription-PCR (RT-PCR) for different arboviruses: DENV, ZIKV and CHIKV;and for the EV; and by PCR for the HHV1-5 complex (HSV1-2, VZV, EBV andCMV). Also, ELISA was carried out in a subgroup of remaining samples fordetection of DENV IgM and NS1 antigen, CHIKV IgM and ZIKV IgM.Results: Thirty-seven patients were PCR or ELISA positive for at least one of thestudied viruses (overall positivity 21.8%). EV was the agent most frequentlydetected (10 cases; 27.0%), along with all four DENV serotypes (10 cases;27.0%); followed by CHIKV (6 cases; 16.2%), ZIKV (6 cases; 16.2%), and Varicellazoster virus (VZV) (1 case; 2.7%). Four cases (10.8%) presented viral co-infectiondetected: DENV1 + CHIKV, DENV1 + EV, DENV4 + ZIKV, and CHIKV + ZIKV.Arboviruses (DENV, CHIKV and ZIKV) accounted for the great majority of cases(26 cases; 70.3%) of all single and co-infections: DENV has been the mostfrequently detected arbovirus (13 cases; 35.1%). Among non-arboviralmeningitis,the most common etiology was the EV (11 cases; 29.7%).Conclusions: Arboviruses accounted for the majority of identified virusesamong patients with suspected viral meningitis. In areas where they areendemic it is crucial to increase viral surveillance and consider them in thedifferential diagnosis of meningitis.Fil: Dias, Tamiris T.. Fundación Oswaldo Cruz; BrasilFil: Tauro, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; ArgentinaFil: Macêdo, Lara E. N.. Fundación Oswaldo Cruz; BrasilFil: Brito, Liz O.. Fundación Oswaldo Cruz; BrasilFil: Ribeiro, Victor H. O.. Fundación Oswaldo Cruz; BrasilFil: Santos, Cleiton S.. Fundación Oswaldo Cruz; BrasilFil: Jacob-Nascimento, Leile C.. Fundación Oswaldo Cruz; BrasilFil: Vilas-Boas, Letícia S.. Fundación Oswaldo Cruz; BrasilFil: Amado, Caio. Fundación Oswaldo Cruz; BrasilFil: Barbosa, Paula S.. Fundación Oswaldo Cruz; BrasilFil: Reis, Joice N.. Fundación Oswaldo Cruz; BrasilFil: Soares Campos, Gubio. Fundación Oswaldo Cruz; BrasilFil: Ribeiro, Guilherme S.. Fundación Oswaldo Cruz; BrasilFil: Siqueira, Isadora C.. Fundación Oswaldo Cruz; BrasilFil: Silva, Luciano K.. Fundación Oswaldo Cruz; BrasilFil: Reis, Mitermayer G.. Fundación Oswaldo Cruz; Brasi

Health policy in emerging economies: Innovations and challenges

Over the last decade, the global health agenda has converged around proposals for the construction of policies and interventions towards greater equity. Despite the great diversity in their historical and institutional contexts, developing countries face similar health care challenges, including inequity regarding access to services and to quality medicines, the prevention and control of communicable and non-communicable diseases, and the strengthening of capacities in scientific and technological development. [...

Current Topics in Neglected Tropical Diseases

Neglected tropical diseases (NTDs) is a diverse group of communicable diseases that prevail in tropical and subtropical conditions in 149 countries. NTDs affect more than one billion people and cost developing economies billions of dollars every year. According to the World Health Organization (WHO), NTDs mainly affect populations living in poverty, without adequate sanitation, and in close contact with infectious vectors, domestic animals, and livestock. Migration, as well as climate change and variability, are key factors in NTD prevalence. Therefore, NTDs deserve more study. Recently, viruses transmitted by vectors (arboviruses) that affect not only people living in the tropics, but also travelers and migrating populations, have been causing epidemics. Examples of these viruses include Dengue, Chikungunya, Zika, Mayaro, and encephalitis viruses. These viruses emerge and reemerge in multiple regions of the world, as occurred in the Americas recently (2013-2017) with Chikungunya and Zika. This book aims to update the significant epidemiological and clinical research of NTDs in many aspects with a multinational perspective

Volume 62-1 Complete Issue

Volume 62-1 Complete Issu

Emerg Infect Dis

PMC4550154611

Environmental Aspects of Zoonotic Diseases

Environmental Aspects of Zoonotic Diseases provides a definitive description, commentary and research needs of environmental aspects related to zoonotic diseases. There are many interrelated connections between the environment and zoonotic diseases such as: water, soil, air and agriculture. The book presents investigations of these connections, with specific reference to environmental processes such as: deforestation, floods, draughts, irrigation practices, soil transfer and their impact on bacterial, viral, fungal, and parasitological spread. Environmental aspects such as climate (tropical, sub-tropical, temperate, arid and semi-arid), developed and undeveloped countries, animal traffic animal border crossing, commercial animal trade, transportation, as well geography and weather on zoonosis, are also discussed and relevant scientific data is condensed and organized in order to give a better picture of interrelationship between the environment and current spread of zoonotic diseases

Rotaviruses and Rotavirus Vaccines

This special edition of Pathogens brings together a breadth of information on rotavirus and global rotavirus vaccines, to highlight rotavirus research from across the world that represents recent advances in our knowledge of vaccine effectiveness, rotavirus epidemiology, genotypic diversity, and genomic characterisation

Abstract Book of the II Congress of the Latin American Society for Vector Ecology

Recopilación de los resúmenes de las conferencias, simposios, paneles de discusión y "turbo talks" ofrecidos en el II Congreso de la Sociedad Latinoamericana de Ecología de Vectores (LA SOVE), realizado entre el 29 de octubre y el 3 de noviembre de 2022 en la ciudad de La Plata (Buenos Aires, Argentina).Sociedad Latinoamericana de Ecología de Vectores (LA SOVE