Micro-CT and mercury intrusion porosimetry characterization of the fabric of roofing slate

The petrological fabric of roofing slate determines the main properties of the slate as a construction material, such as durability and waterproofing. Roofing slates are rocks derived from the low-grade metamorphism of lutites, with a characteristic lepidoblastic fabric. However, little attention by petrologists has been paid to the role of this fabric in the behaviour of roofin slates. This work characterizes the fabric and pore system of two roofing slate varieties from Spain, using two different techniques, each with its advantages and disadvantages: X-ray microtomography, useful to find heterogeneities and mineral inclusions on the slate bulk,and mercury intrusion porosimetry, which defines the pore system. The differentiation of mineral inclusions is very useful for predicting the weathering of a slate, while the definition of the pore system may help to understand how the slate will behave during its service life

Simple sequence repeats in zebra finch (Taeniopygia guttata) expressed sequence tags: a new resource for evolutionary genetic studies of passerines

Background Passerines (perching birds) are widely studied across many biological disciplines including ecology, population biology, neurobiology, behavioural ecology and evolutionary biology. However, understanding the molecular basis of relevant traits is hampered by the paucity of passerine genomics tools. Efforts to address this problem are underway, and the zebra finch (Taeniopygia guttata) will be the first passerine to have its genome sequenced. Here we describe a bioinformatic analysis of zebra finch expressed sequence tag (EST) Genbank entries. Results A total of 48,862 ESTs were downloaded from GenBank and assembled into contigs, representing an estimated 17,404 unique sequences. The unique sequence set contained 638 simple sequence repeats (SSRs) or microsatellites of length ≥20 bp and purity ≥90% and 144 simple sequence repeats of length ≥30 bp. A chromosomal location for the majority of SSRs was predicted by BLASTing against assembly 2.1 of the chicken genome sequence. The relative exonic location (5' untranslated region, coding region or 3' untranslated region) was predicted for 218 of the SSRs, by BLAST search against the ENSEMBL chicken peptide database. Ten loci were examined for polymorphism in two zebra finch populations and two populations of a distantly related passerine, the house sparrow Passer domesticus. Linkage was confirmed for four loci that were predicted to reside on the passerine homologue of chicken chromosome 7. Conclusion We show that SSRs are abundant within zebra finch ESTs, and that their genomic location can be predicted from sequence similarity with the assembled chicken genome sequence. We demonstrate that a useful proportion of zebra finch EST-SSRs are likely to be polymorphic, and that they can be used to build a linkage map. Finally, we show that many zebra finch EST-SSRs are likely to be useful in evolutionary genetic studies of other passerines

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