Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System.

The petals of the saffron crocus (Crocus sativus L.) are considered a waste material in saffron production, but may be a sustainable source of natural biologically active substances of nutraceutical interest. The aim of this work was to study the cardiovascular effects of kaempferol and crocin extracted from saffron petals. The antiarrhythmic, inotropic, and chronotropic effects of saffron petal extract (SPE), kaempferol, and crocin were evaluated through in vitro biological assays. The antioxidant activity of kaempferol and crocin was investigated through the 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) assay using rat cardiomyoblast cell line H9c2. The MTT assay was applied to assess the effects of kaempferol and crocin on cell viability. SPE showed weak negative inotropic and chronotropic intrinsic activities but a significant intrinsic activity on smooth muscle with a potency on the ileum greater than on the aorta: EC50 = 0.66 mg/mL versus EC50 = 1.45 mg/mL. Kaempferol and crocin showed a selective negative inotropic activity. In addition, kaempferol decreased the contraction induced by KCl (80 mM) in guinea pig aortic and ileal strips, while crocin had no effect. Furthermore, following oxidative stress, both crocin and kaempferol decreased intracellular ROS formation and increased cell viability in a concentration-dependent manner. The results indicate that SPE, a by-product of saffron cultivation, may represent a good source of phytochemicals with a potential application in the prevention of cardiovascular diseases

Long-term oral administration of sweet chestnut (Castanea sativa Mill.) extract does not affect the contraction ability of isolated ileum

Extracts of sweet chestnut (Castanea sativa Mill.) which contain hydrolysable tannins are used to prevent diarrhoea in farm animals due to the astringent effect of tannins. The aim of this study was to establish whether long-term treatment with the chestnut extract as a feed additive affects the acetylcholine (ACh)-triggered contractions of isolated rat ileum. Wistar rats of both sexes were divided into 4 male and 4 female groups with 6 animals in each group. The first, second and third groups of both sexes received feed with 3, 15 and 30 g of chestnut extract per kg, respectively. The fourth group of both sexes received feed without the added chestnut extract and served as the control group. After the 90-day trial, the animals were euthanized, and ilea were isolated and placed in an organ bath filled with Tyrode’s solution. Ilea were connected to an isometric transducer. Ileal contractions were triggered by 0.005, 0.01, 0.05, 0.1, 0.5, 1.0, 5.0 and 10.0 mg of ACh, and contractions were recorded. The groups exhibited dose responsiveness to the ACh treatment steps; however, no significant differences in the contraction intensity were observed among the groups at the same ACh dose level. These results suggest that long-term treatment with chestnut extract as an astringent does not influence the intensity of ileal contractions triggered by ACh. Thus, no adverse effect on intestinal motility can be expected during a long-term use of chestnut extract as a feed additive in livestock production; however, further studies in target animal species are needed to confirm the findings.</jats:p

Biocomposites

Biocomposites are composite materials consisting of either a polymer matrix or a filler based on biological resources. They have been widely used in numerous applications such as storage devices, photocatalysts, packaging, furniture, biosensors, energy, construction, the automotive industry, and so on due to their great versatility and satisfactory performance. This book focuses on composites made from natural materials (natural fibers and biopolymers) and relates their physical, mechanical, electrical, structural, and biological characteristics as well as their potential applications in biomedicine, pharmaceuticals, and engineering

WEED INFESTATION OF WINTER WHEAT IN DIFFERENT TILLAGE SYSTEMS AND LEVEL OF NITROGEN IN TOP DRESSING

Growing technology, especially tillage and fertilization of economically important crop species such as wheat, plays a very important role in weed control. Successful weed control in the crop in turn significantly affects the formation of grain yield, both in quantity and quality. The aim of this paper was to investigate the influence of sustainable (mulch - and no- tillage) and conventional farming system on weed infestation of winter wheat. Basic fertilization was uniform (600 kg/ha NPK 15:15:15) while weed infestation differences between three levels of nitrogen fertilization in top dressing (0, 60 and 120 kg/ha) were examined. The variety Pobeda, selected at the Institute of Field and Vegetable Crops in Novi Sad, served as the object of investigation. The examination was performed at "Radmilovac" on the experimental school property of the Faculty of Agriculture in Zemun within the four- crop rotation (maize-winter wheat-spring barley + red clover-red clover) on leached chernozem soil type in a two-year period. The system of conventional tillage showed the highest efficiency in the weed control (number of weed species and number of weed plants per species) of the two conservation systems. The next is the system of mulch tillage, which may be of interest for practice, while the system of no tillage had the lowest efficiency in the control of weeds, especially perennials. Increasing the amount of nitrogen in the top dressing reduces weeds in all tillage systems, mainly due to the stronger competitiveness of winter wheat. The highest fresh biomass of weeds was measured in the no-tillage system (especially in the second year of investigation) due to the significantly higher presence of perennial broadleaf weeds

Food science sourcebook

2 v. : ill. ; 26 cm2nd edition.Rev. ed. of: Source book for food scientists. c1978"An AVI book."Pt. 1. Terms and descriptions -- pt. 2. Food composition,properties, and general data

Variability of tillering in winter wheat (Triticum aestivum L.)

Variability of length of spike and number of spikelets spike-1 have share in forming of grain yield of wheat. The aim of this study was estimation of variability of length of spike and number of spikelets spike-1 in 20 genetically divergent wheat cultivars grown in different environmental conditions. The experiment was set up as a randomised block design in three replications. Obtained results indicated differences in average values of length of spike and number of spikelets spike-1 among tested cultivars in both years of experiment. In average for all cultivars length of spike was higher in the second year than in first year of experiment. Also, average value of number of spikelets spike-1 was higher in second year at the analysed wheat cultivars. The wheat cultivar Dejana expressed the highest length of spike (12.50cm) in average in the second experimental year while the wheat cultivar Sumadinka had the least length of spike (8.91cm) in average in the first year. Based on the results was established, variability of wheat cultivars for the both analysed traits of spike, as well as, significant differences between the wheat cultivars according to length of spike and number of spikelets spike-1 , which are in dependence of genetic and environmental factors