Development of somatic cell nuclear transfer biotechnology for cloning of animals: a mini-review

Somatic cell nuclear transfer (SCNT) in cloning animals is important for reproductive biotechnology, it can be used for the conservation of wild, endangered or critically endangered, and also to improve superior local livestock genetic resources. So many publications that show the success of animal cloning with SCNT for various purposes, as well as the development of animal cloning methods. This paper will attempt to review the results of successful and limited animal cloning research on the needs of non-threatened (livestock and wild animals) and threatened wildlife. This research was kind of literature review. The research was conduct several phase these are identification, abstract screening, full-text selection, and mini-review writing. The results of SCNT biotechnology for animal cloning can be used for the preservation of wild animal germplasm genetic resources, increasing the production of superior livestock and ways of conserving threatened animals

Agricultural and Biomedical Application of Animal Cloning: Review

Cloning is a creation of an organism which is called clone that that is a genetic copy of another organism or the donor by using different technique such as somatic cell nuclear transfer and embryo splitting. The advanced applications of cloning are biomedical applications which comprehend research model, bioreactors, and xenotransplantation. Rapid multiplication of desirable livestock and conservation of gene pools are enclosed under agricultural application. Particularly Cloning has the potential to improve the efficiency of trans-genesis in these applications, as well as a role for the multiplication of animals of proven production. In developing country science is not yet conceived and the concerned body should pay attention to such valuable aspects of biotechnological advancement. Keywords: Bioreactors, Cloning, Embryo Splitting, Gene Pool

Establishment of pluripotent cell lines from vertebrate species - Present status and future prospects

Pluripotent embryonic stem (ES) cells are undifferentiated cell lines derived from early embryos and are capable of unlimited undifferentiated proliferation in vitro. They retain the ability to differentiate into all cell types including germ cells in chimeric animals in vivo, and can be induced to form derivatives of all three germ layers in vitro. Mouse ES cells represent one of the most important tools in genetic research. Major applications include the targeted mutation of specific genes by homologous recombination and the discovery of new genes by gene trap strategies. These applications would be of high interest for other model organisms and also for livestock species, However, in spite of tremendous research activities, no proven ES cells colonizing the germ line have been established for vertebrate species other than mouse a nd chicken thus far. This review summarizes the current status of deriving pluripotent embryonic stem cell lines from vertebrates and recent developments in nuclear transfer technology, which may provide an alternative tool for genetic modification of livestock animals. Copyright (C) 1999 S. Karger AG, Basel

Vision of breeding for organic agriculture

This report describes the results of research into the vision of breeding systems within organic agriculture in the Netherlands. The purpose of the research was to arrive at a vision for breeding in organic agriculture by means of interviews and discussions with organic livestock farmers and social organisations. The research was prompted by the fact that, as things stand, organic livestock farmers generally have to rely on the conventional breeding supply. Neither the breeding method not the animal type produced meet the requirements of organic agriculture. Interest in breeding has increased because organic agriculture is expanding, and as yet too little attention has been paid to the development of specific organic breeding programmes and associated legislation. In recent decades conventional agriculture and breeding have tended more and more towards industrialisation and uniformity with breeding becoming a multinational concern. The breeding organisations have no incentive at present to provide special services for organic agriculture as the market is too small and the costs are too high. Taking the current breeding situation as the starting point, a number of scenarios are described for each animal sector which could gradually lead to a system of breeding which is more organic both in its aims and in the chain-based approach. The naturalness of the breeding techniques is an important factor in considering the available options. The different scenarios served as a basis for the interviews and discussions with livestock farmers and social organisations. We have established that the farmers and organisations consider it important for organic agriculture to work on developing a breeding system which follows the principles of organic agriculture. The most important reasons for this are that: (1) consumers expect all production factors in the chain to be of organic origin, (2) most livestock farmers currently use conventional breeding techniques which fall short of the organic requirements on a number of points, such as the use of artificial reproduction techniques and mono-functional breeding for production. For the development of organic breeding, livestock farmers feel that in the first instance the use of artificial reproduction techniques, including cloning and transgenic techniques, should be restricted. Next the livestock farmers and social organisations want breeding to be adapted to or based on the organic environment. There is a suspicion that owing to genotype-environment interaction (G x E) conventionally-bred animals cannot adapt well to the organic environment, and this leads to health and welfare problems. The farmers would like to see this development taking place within 5 to 10 years. It must however proceed one step at a time since the farmers cannot yet form a complete picture of the impact of all the different factors. Most of the people involved see the ideal form of breeding, with natural reproduction and regional or farm-specific selection, as a standard to be achieved in the distant future. At the moment most livestock farmers have neither the knowledge nor the socio-economic means to set up such breeding programmes. The development of breeding and the associated legislation require an international approach, for which suitable contacts must be sought in other countries. The final chapter of this report looks in more detail at the steps to be taken in each sector. Ideally developments should probably be initiated and supervised by a central body, such as an organic breeding foundation, which could be set up to govern the breeding of all farm animals

Consumer Preferences for Amount and Type of Fat in Ground Beef

Scientists and beef industry participants are investigating ways to improve the healthiness of beef. We report results of a nationwide mail survey developed to determine consumers’ preferences for fat content in ground beef and identify how consumers would most like to improve the healthiness of beef. The results from a choice-based conjoint experiment indicate that consumers place significant value on reducing saturated fat and the Omega 6:3 ratio in ground beef, but were relatively unconcerned about conjugated linoleic acid. The relatively new method of best-worst scaling was used to further identify which methods consumers most preferred producers use to improve fat content in beef. The results indicate consumers preferred feeding cattle a grass-fed diet as opposed to supplementing cattle feed with fishmeal or flaxseed to improve the fatty acid content in beef. Although consumers were receptive to the idea of using genetic testing to breed only those cattle with improved fatty acid content, using cloning to achieve this end, was viewed as very undesirable.beef, best-worst scaling, cloning, conjoint, fat, maximum-difference scaling, omega 3 fatty acid, Agribusiness, Demand and Price Analysis, Food Consumption/Nutrition/Food Safety, Livestock Production/Industries, Marketing, M31, Q13,

The genomes and history of domestic animals

This paper reviews how mammalian genomes are utilized in modern genetics for the detection of genes and polymorphisms (mutations) within domesticated animal (mostly livestock) genomes that are related to traits of economic importance to humans. Examples are given of how genetic analysis allows to determine key genes associated with the quality and quantity of milk in cattle and key genes for meat production. Various questions are reviewed, such as how contemporary methods of genome sequencing allow to maximise the effective detection of coding and regulatory DNA polymorphisms within the genomes of major domesticated mammals (cattle, sheep and pigs) and the history of their formation from the standpoint of genetics

The astacin metalloprotease moulting enzyme NAS-36 is required for normal cuticle ecdysis in free-living and parasitic nematodes

Nematodes represent one of the most abundant and species-rich groups of animals on the planet, with parasitic species causing chronic, debilitating infections in both livestock and humans worldwide. The prevalence and success of the nematodes is a direct consequence of the exceptionally protective properties of their cuticle. The synthesis of this cuticle is a complex multi-step process, which is repeated 4 times from hatchling to adult and has been investigated in detail in the free-living nematode, Caenorhabditis elegans. This process is known as moulting and involves numerous enzymes in the synthesis and degradation of the collagenous matrix. The nas-36 and nas-37 genes in C. elegans encode functionally conserved enzymes of the astacin metalloprotease family which, when mutated, result in a phenotype associated with the late-stage moulting defects, namely the inability to remove the preceding cuticle. Extensive genome searches in the gastrointestinal nematode of sheep, Haemonchus contortus, and in the filarial nematode of humans, Brugia malayi, identified NAS-36 but not NAS-37 homologues. Significantly, the nas-36 gene from B. malayi could successfully complement the moult defects associated with C. elegans nas-36, nas-37 and nas-36/nas-37 double mutants, suggesting a conserved function for NAS-36 between these diverse nematode species. This conservation between species was further indicated when the recombinant enzymes demonstrated a similar range of inhibitable metalloprotease activities

Generation of cloned transgenic pigs rich in omega-3 fatty acids

Meat products are generally low in omega-3 (n-3) fatty acids, which are beneficial to human health. We describe the generation of cloned pigs that express a humanized Caenorhabditis elegans gene, fat-1, encoding an n-3 fatty acid desaturase. The hfat-1 transgenic pigs produce high levels of n-3 fatty acids from n-6 analogs, and their tissues have a significantly reduced ratio of n-6/n-3 fatty acids (P < 0.001). © 2006 Nature Publishing Group