Generation of Transplantable Retinal Photoreceptors from a Current Good Manufacturing Practice-Manufactured Human Induced Pluripotent Stem Cell Line.

Retinal degeneration often results in the loss of light-sensing photoreceptors, which leads to permanent vision loss. Generating transplantable retinal photoreceptors using human somatic cell-derived induced pluripotent stem cells (iPSCs) holds promise to treat a variety of retinal degenerative diseases by replacing the damaged or dysfunctional native photoreceptors with healthy and functional ones. Establishment of effective methods to produce retinal cells including photoreceptors in chemically defined conditions using current Good Manufacturing Practice (cGMP)-manufactured human iPSC lines is critical for advancing cell replacement therapy to the clinic. In this study, we used a human iPSC line (NCL-1) derived under cGMP-compliant conditions from CD34+ cord blood cells. The cells were differentiated into retinal cells using a small molecule-based retinal induction protocol. We show that retinal cells including photoreceptors, retinal pigmented epithelial cells and optic cup-like retinal organoids can be generated from the NCL-1 iPSC line. Additionally, we show that following subretinal transplantation into immunodeficient host mouse eyes, retinal cells successfully integrated into the photoreceptor layer and developed into mature photoreceptors. This study provides strong evidence that transplantable photoreceptors can be generated from a cGMP-manufactured human iPSC line for clinical applications. Stem Cells Translational Medicine 2018;7:210-219

Effect of packaging materials on the chemical composition and microbiological quality of edible mushroom (Pleurotus ostreatus) grown on cassava peels

Edible fungi such as mushrooms are highly perishable and deteriorate few days after harvest due to its high moisture content and inability to maintain their physiological status. In this study, the effect of packaging materials on the nutritional composition of mushroom cultivated from cassava peels was investigated. Mushroom samples were dried at 50°C in a cabinet dryer for 8 h. The dried mushroom samples packaged in four different packaging materials; high density polyethylene (HDPE), polypropylene (PP), laminated aluminum foil (LAF), high density polyethylene under vacuum (HDPEV) were stored at freezing (0°C) temperatures for 12 weeks. Samples were collected at 2-week intervals and analyzed for proximate composition (carbohydrate, protein, fat, fiber, ash, moisture), mineral content (calcium, potassium), vitamin C content, and microbiological qualities (total aerobic count, Pseudomonal count, Coliform count, Staphylococcal count, Salmonella count) using the standard laboratory procedures. Carbohydrate, protein, fat content of dried mushrooms packaged in HDPE at freezing temperature ranged from 45.2% to 53.5%, 18.0% to 20.3%, and 3.2% to 4.3%, while mushrooms in polypropylene ranged from 45.2% to 53.5%, 18.5% to 20.3%, 2.6% to 4.3%. Carbohydrate, protein, fat of mushroom in LAF ranged from 47.8% to 53.5%, 17.3% to 20.3%, and 3.3% to 4.3%, respectively, while carbohydrate, protein, fat of mushroom in HDPEV ranged from 51.1% to 53.5%, 19.5% to 20.3%, and 3.5% to 4.3%. Microbiological analysis showed that total aerobic count, Pseudomonal count, and Staphyloccocal count of dried mushroom ranged from 2.3 to 3.8 log cfu/g, 0.6 to 1.1 log cfu/g, and 0.4 to 0.5 log cfu/g, respectively. In conclusion, dried mushroom in HDPE packaged under vacuum at freezing temperature retained the nutritional constituents than those packaged with other packaging materials

Heat Sealability Of Laminated Films With LLDPE And LDPE As The Sealant Materials In Bar Sealing Application

The heat sealability of laminated films with linear low density polyethylene (LLDPE) and low density polyethylene (LDPE) as the sealant materials was investigated. A laboratory heat sealer was used to study the response of laminated films to temperature, time, and pressure. Platen temperature was confirmed as primary factor in controlling heat-seal strength. Dwell time must be sufficiently long to bring the interfacial temperature to a desired level. When the desired heat-seal strength has been achieved, further increase of dwell time did not improved heat-seal strength. Platen pressure had little effect above the level required to flatten the materials for good contact. Bar sealing process window for each sample were developed. The optimum combination of platen temperature and dwell time for each laminated film can be obtained in the respective process windows. Strength of heat-seal and its failure modes are closely related. Plateau initiation temperature closely corresponds to the final melting point of sealant materials. Relatively higher platen temperature was required to seal laminated films with lower thermal conductance. Required dwell time corresponds closely to the heat flow rate of bar sealing process. Laminated films made from extrusion lamination process provided lower level of achievable heat seal strength when compared with the laminated films made from dry-bond lamination process

Effect of design parameters on thermal performance of integrated phase change material blind system for double skin façade buildings

Double skin facades (DSFs) have overheating problems in warm seasons which may increase the cooling loads in buildings. A previous study has developed an integrated phase change material (PCM) blind system and proved its capacity of mitigating the overheating phenomenon in DSFs. This paper focuses on the effect of design parameters on the thermal performance of such systems by conducting a simulation study of a DSF integrated with a PCM blind with different material properties, positions in cavity, and tilt angles of blades. The results indicate that the performance of the integrated PCM blind system can be optimised with careful geometric design and proper thermophysical properties of the PCM

Human induced pluripotent stem cells generate light responsive retinal organoids with variable and nutrient dependent efficiency

The availability of in vitro models of the human retina in which to perform pharmacological and toxicological studies is an urgent and unmet need. An essential step for developing in vitro models of human retina is the ability to generate laminated, physiologically functional and light-responsive retinal organoids from renewable and patient specific sources. We investigated five different human induced pluripotent stem cell (iPSC) lines and showed a significant variability in their efficiency to generate retinal organoids. Despite this variability, by month 5 of differentiation, all iPSC-derived retinal organoids were able to generate light responses, albeit immature, comparable to the earliest light responses recorded from the neonatal mouse retina, close to the period of eye opening. All iPSC-derived retinal organoids exhibited at this time a well-formed outer nuclear like layer containing photoreceptors with inner segments, connecting cilium and outer like segments. The differentiation process was highly dependent on seeding cell density and nutrient availability determined by factorial experimental design. We adopted the differentiation protocol to a multiwell plate format which enhanced generation of retinal organoids with retinal pigmented epithelium (RPE) and improved ganglion cell development and the response to physiological stimuli. We tested the response of iPSC-derived retinal organoids to Moxifloxacin and showed that similarly to in vivo adult mouse retina, the primary affected cell types were photoreceptors. Together our data indicate that light responsive retinal organoids derived from carefully selected and differentiation efficient iPSC lines can be generated at the scale needed for pharmacology and drug screening purposes. © AlphaMed Press 2018

Model Production System for Laminated Wood Products

The purpose of this research was to investigate and experiment with the use of a designed model production system in comparison to a traditional system. It was the intent of this study to develop an efficient model production system for producing laminated wood products. This research included: (l) review of the literature pertinent to wood lamination, (2) survey of companies and individuals involved with wood lamination, (3) design and testing of a model lamination system, and (4) comparison of the designed system to the traditional method of laminating a wood product The variables and procedures relating to wood lamination were identified through the review of literature and returned surveys. The variables and procedures deemed important were used to design the schematic, mathematical, and physical models of a lamination system. The designed model system was compared to the traditional method of producing a laminated wood product. This was done through a comparison of production times, process charts, process layout diagrams, and cost analysis The primary conclusions obtained from this study were: (1) the designed model layout resulted in less material movement than the traditional layout, (2) the designed model process required fewer production activities than the traditional process, (3) the designed model process required less production time than the traditional method, (4) the designed model proved to be more economical in producing the same quantity of products than the traditional method, and (5) the quality of the laminated wood product proved higher using the designed model system in comparison to the traditional method It is recommended that: (1) a designed layout be used in lamination production runs, (2) a designed process should be incorporated in lamination production runs, (3) break-even analysis should be used to compare the economics of two production methods, and (4) models should be used to compare two or more competing system

Geographic Concentration in U.S. Manufacturing Industries: A Dartboard Approach

This paper discusses the prevalence of Silicon Valley-style localizations of individual manufacturing industries in the United States. Several models in which firms choose locations by throwing darts at a map are used to test whether the degree of localization is greater than would be expected to arise randomly and to motivate a new index of geographic concentration. The proposed index controls for differences in the size distribution of plants and for differences in the size of the geographic areas for which data is available. As a consequence, comparisons of the degree of geographic concentration across industries can be made with more confidence. We reaffirm previous observations in finding that almost all industries are localized, although the degree of localization appears to be slight in about half of the industries in our sample. We explore the nature of agglomerative forces in describing patterns of concentration, the geographic scope of localization, and the extent to which agglomerations involve plants in similar as opposed to identical industries.

The Restoration of Central City Alvar Aalto Library in Vyborg

The restoration of the Central City Alvar Aalto Library in Vyborg is completed and was officially inaugurated on 23rd November 2013. The restoration has been a long process which started in 1991. The work was carried out as a Russian–Finnish joint cross–border project within the context of two different socio–cultural societies, customs difficulties, economic fluctuations and currency rates, which could change the situation overnight. The project has been a learning process for all who have participated during the past years

Bamboo, Hope for the Wood Industry in Ghana

Over exploitation of some of the forest products in Ghana has led to their extermination. The current state of the Ghanaian woodland has drawn Government attention to wood sources that are renewable, environmentally supportive and fast growing. This is an attempt to check deforestation and to develop alternative resource for the fast dyeing wood industry which depends on the forest for its supply of timber? Studies made on bamboo, uncovered its capabilities and potentials as an appropriate material for adoption to replace Timber wood. Keywords: Bamboo, forest, extinction, timber wood, lamination, deforestatio