RNA interference in marine and freshwater sponges

Background: The marine sponge Tethya wilhelma and the freshwater sponge Ephydatia muelleri are emerging model organisms to study evolution, gene regulation, development, and physiology in non-bilaterian animal systems. Thus far, functional methods (i.e., loss or gain of function) for these organisms have not been available. Results: We show that soaking developing freshwater sponges in double-stranded RNA and/or feeding marine and freshwater sponges bacteria expressing double-stranded RNA can lead to RNA interference and reduction of targeted transcript levels. These methods, first utilized in C. elegans, have been adapted for the development and feeding style of easily cultured marine and freshwater poriferans. We demonstrate phenotypic changes result from ‘knocking down’ expression of the actin gene. Conclusion: This technique provides an easy, efficient loss-of-function manipulation for developmental and gene regulatory studies in these important non-bilaterian animals

Fish and freshwater crayfish communities of the Brunswick and Preston Rivers

The Leschenault Inlet, located on the Swan Coastal Plain near Bunbury, is the estuary of the Brunswick, Collie, Preston and Ferguson Rivers. There is a considerable amount of information detailing the fish fauna of the Leschenault Inlet with Potter et al. (1997, 2000) recording 42 species of fish from 26 families. The estuary is dominated by gobies, atherinids and clupeids which comprised almost 90% of the fishes. Potter et al. (2000) also reported 13 species from gill net captures in the lower (tidal) Collie River. Neither of these studies reported on the fishes found within the freshwaters of the Leschenault Inlet catchment and although Pen & Potter (1990, 1991a, b, c, d, 1992) conducted biological studies on a number of native and introduced freshwater fishes in the upper Collie River by, there is no published information regarding the prevailing fishes (or freshwater crayfishes) of the Brunswick or Preston Rivers. Hale et al. (2000) reported on the fish fauna of one site in the upper Brunswick River, near the confluence with the Augustus River, one site in the Augustus River and two sites in the Ernest River. While they recorded only two species of freshwater fish from the Ernest River (i.e. Western Pygmy Perch (Edelia vittata) and Nightfish (Bostockia porosa)), they captured a further two endemic freshwater fishes from the Augustus and upper Brunswick Rivers (i.e. Western Minnow (Galaxias occidentalis) and Freshwater Cobbler (Tandanus bostocki)) as well as larval (ammocoetes) Pouched Lampreys (Geotria australis) and three introduced fishes (i.e. Rainbow Trout (Oncorhynchus mykiss), Brown Trout (Salmo trutta) and Eastern Mosquitofish (Gambusia holbrooki)). The presence of the larger introduced fishes, the lamprey and the Freshwater Cobbler in the Augustus River compared to the Ernest River was attributed to the provision of environmental water releases from Freshwater Lake. These species are more often associated with permanent waters over ephemeral systems. The distributions of fishes inhabiting the inland waters of the south-western corner of Western Australia were documented in Morgan et al. (1998) and include the systems from Capel to Two People’s Bay east of Albany and thus does not encompass the more northerly Leschenault catchments, but does include some sites on the Collie River South Branch. The aims of the present study were to ameliorate the paucity of knowledge of the fishes and freshwater crayfishes inhabiting two of the major systems flowing into the Leschenault Inlet and thereby provide the Leschenault Catchment Council with an overview of the fishes of the Brunswick and Preston Rivers

Characterisation of host growth after infection with a broad-range freshwater cyanopodophage

Freshwater cyanophages are poorly characterised in comparison to their marine counterparts, however, the level of genetic diversity that exists in freshwater cyanophage communities is likely to exceed that found in marine environments, due to the habitat heterogeneity within freshwater systems. Many cyanophages are specialists, infecting a single host species or strain; however, some are less fastidious and infect a number of different host genotypes within the same species or even hosts from different genera. Few instances of host growth characterisation after infection by broad host-range phages have been described. Here we provide an initial characterisation of interactions between a cyanophage isolated from a freshwater fishing lake in the south of England and its hosts. Designated ΦMHI42, the phage is able to infect isolates from two genera of freshwater cyanobacteria, Planktothrix and Microcystis. Transmission Electron Microscopy and Atomic Force Microscopy indicate that ΦMHI42 is a member of the Podoviridae, albeit with a larger than expected capsid. The kinetics of host growth after infection with ΦMHI42 differed across host genera, species and strains in a way that was not related to the growth rate of the uninfected host. To our knowledge, this is the first characterisation of the growth of cyanobacteria in the presence of a broad host-range freshwater cyanophage

Fishing systems: viability in Nigeria

Following an outline of the various fishing systems in Nigeria, considering both the marine and freshwater environment, details are provided of those around which viable sustainable fisheries may be develope

Groundwater ages, recharge conditions and hydrochemical evolution of a barrier island freshwater lens (Spiekeroog, Northern Germany)

Freshwater lenses below barrier islands are dynamic systems affected by changes in morphodynamic patterns, groundwater recharge and discharge. They are also vulnerable to pollution and overabstraction of groundwater. Basic knowledge on hydrogeological and hydrochemical processes of freshwater lenses is important to ensure a sustainable water management, especially when taking into account possible effects of climate change. This is the first study which gives a compact overview on the age distribution, recharge conditions and hydrochemical evolution of a barrier island freshwater lens in the southern North Sea (Spiekeroog Island, Eastfrisian Wadden Sea). Two ground- and surface water sampling campaigns were carried out in May and July 2011, supplemented by monthly precipitation sampling from July to October. 3H–3He ages, stable oxygen and hydrogen isotopes and major ion concentrations show that the freshwater lens reaches a depth of 44 mbsl, where an aquitard constrains further expansion in vertical direction. Groundwater ages are increasing from 4.4 years in 12 mbsl up to >70 years at the freshwater– saltwater interface. Stable isotope signatures reflect average local precipitation signatures. An annual recharge rate of 300–400 mm was calculated with 3H–3He data. Freshwater is primarily of Na–Ca–Mg–HCO3– and Ca–Na–HCO3–Cl type, while lowly mineralized precipitation and saltwater are of Na–Cl types. A trend towards heavier stable isotope signatures and higher electric conductivities in the shallower, younger groundwater within the freshwater lens may indicate increasing atmospheric temperatures in the last 30 years

Toxicokinetics and bioconcentration of polycyclic aromatic hydrocarbons in freshwater isopods

A novel method based on a first-order two-compartment model was used to determine the bioconcentration and toxicokinetic rate constants of six different polycyclic aromatic hydrocarbons (PAHs) in the freshwater isopod Asellus aquaticus, a common species in most European freshwater systems. Numerical integration and iterative parameter estimation techniques were applied to account for time-varying aqueous exposure concentrations. All PAHs exhibited a rapid uptake. Monophasic elimination patterns were observed for benzo[e]pyrene and benzo[a]-pyrene (biological half-life

Freshwater Culture Of Salmonids In Recirculating Aquaculture Systems (RAS) With Emphasis On The Monitoring And Control Of Key Environmental Parameters

This report is intended as a briefing paper on Recirculating Aquaculture Systems with emphasis on the monitoring of water quality parameters relating to the freshwater culture of the Arctic charr Salvelinus alpinus

Ascending the Avon: fishes of the Northam Pool, and the Swan-Avon catchment

The fish fauna in the vicinity of the Northam Pool Weir was sampled seasonally between winter 2008 and autumn 2009. The results indicate that the fish community was characterised by species that are halotolerant including two estuarine species, the Western Hardyhead and Swan River Goby that are likely to have undergone large upstream expansions in the Swan‐Avon catchment due to secondary salinisation. However, two freshwater endemic species, the Western Minnow and Nightfish were also recorded in the vicinity of the weir. These, and other freshwater endemic species, have undergone large range reductions in this catchment as a result of salinisation. The study found evidence that the weir may be impeding the upstream movements of native fishes as found elsewhere in south‐western Australia and that construction of a well‐designed fishway would enhance population connectivity and increase their sustainability. It is recommended that additional sampling occurs during the major spawning periods of the freshwater species immediately below the weir to determine precisely when a future fishway would need to operate to allow maximum usage by resident native species. It is also recommended that fresh refuge habitats for freshwater fishes be identified to allow effective management measures to be implemented in those systems to halt their decline and reduce the risk of complete loss of these species from the Swan‐Avon catchment

Freshwater fish and crayfish communities of the tributaries of the Margaret River

Tributaries and headwaters of major rivers are known to be important spawning and nursery habitats of freshwater endemic fishes in south-western Australia (see for example the Collie River in Pen & Potter 1990, and the Blackwood River in Beatty et al. 2006, 2008). Fishes of the Margaret River have previously been examined by Morgan et al. (1998) and Morgan & Beatty (2003) with the monitoring of the functioning of the two fishways on the river documented in Morgan & Beatty (2004, 2007) and Beatty & Morgan (2008). The river is known to be of conservation importance due to it housing five of the eight endemic freshwater fishes of the south-west region, as well as housing the majority (five of the six species) of the Cherax species of freshwater crayfishes found in the south-west; including the Margaret River endemic Critically Endangered Hairy Marron. Despite this known value and considerable volume of research on the fishes in the main channel of the Margaret River, little is known on the fishes and freshwater crayfishes of the river 19s major tributaries. The aim of this study is to document the freshwater fish distribution in the major tributaries of the Margaret River (i.e. Bramley, Darch, and Yalgardup Brooks) during or close to the breeding period for the majority of the species and to provide a broad assessment and comparison of population demographics of the different species in the different tributaries. This information is required for the formulation of River Action Plans for these systems by the Cape to Cape Catchments Group