1,587 research outputs found

    PHYTOCHEMICAL SCREENING AND HPTLC FINGERPRINTING ANALYSIS OF ETHANOLIC EXTRACT OF ERYTHRINA VARIEGATA L. FLOWERS

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    Objective: Medicinal plants possess phytochemicals that accumulate in various parts of plant like leaves, flowers, vegetables and roots that exert defense mechanism and protect us from various diseases. The present study was carried out to investigate the secondary metabolites and develop HPTLC fingerprinting of Erythrina variegata L.Methods: The Phytochemical screening was analyzed for various extracts by using standard protocols and HPTLC analysis was carried out for the identification of alkaloids, flavonoids, glycosides, saponin and steroids in the ethanolic extract of Erythrina variegata L. flower.Results: The phytochemicals screening confirms the presence of phyto-constituents in various plant extracts of Erythrina variegata L. The study revealed the presence of alkaloids, flavonoids, glycosides, saponin and steroids in the ethanolic extract of Erythrina variegata L. flower.Conclusion: Based on the study it is concluded that Erythrina variegata L. flower possess phytochemicals like alkaloids, flavonoids, glycosides, saponin and steroids.Keywords: Erythrina variegata L., HPTLC, Medicinal plants, Phytoconstituents, Secondary metabolite

    Erythrina foliage as an alternative feed for growing goats in Lao PDR

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    Foliages from some tropical plants and from different species of the legume Erythrina were examined in five studies assessing the characteristics of these foliages as feed for goats. Digestibility and feed preference studies showed that ad libitum feeding of foliage from Erythrina (Erythrina variegata), jackfruit (Artocarpus heterophyllus) and kapok (Ceiba pentandra) resulted in a longer time spent eating and more changes in diet selection, feed intake, crude protein (CP) digestibility and nitrogen (N) retention than foliage from jujube (Ziziphus jujube Mill), fig (Ficus racemosa) and mango (Mangifera indica). Supplementation of low quality foliage with water spinach (Ipomoea aquatica) increased intake and apparent digestibility. In a survey study, three different Erythrina spp., E. indica, E. subumbrans and E. variegata, were found in the north, centre and south of Lao PDR. These species were in limited use as an animal feed on smallholder farms. Erythrina variegata appeared to be a better option in terms of degradability than E. indica and E. subumbrans according to in vitro gas production and in sacco degradation studies. A cropping study showed that cow manure or mineral fertiliser significantly decreased shoot mortality and increased shoot height and fresh and CP yield of E. variegata foliage compared with no fertiliser. The nutritive value of the foliage did not differ significantly between the fertilised and unfertilised treatments. Studies on different degrees of processing (fresh or sun-dried) of E. variegata foliage showed similar CP, neutral detergent fibre and acid detergent fibre contents, and no difference in total voluntary intake, when either fresh or dry foliage was fed ad libitum to growing goats. Four levels of replacement (0, 20, 40 and 60%) of dietary CP with CP from E. variegata foliage resulted in similar dry matter intake, apparent CP digestibility, N retention and carcass characteristics, but total CP intake was significantly higher in goats fed the diet with 60% replacement compared with 20%. Thus CP from E. variegata foliage can replace up to 60% of CP in a mixed diet with soybean meal without any negative effects on animal growth performance

    ANTI INFLAMMATORY ACTIVITY OF ERYTHRINA VARIEGATA

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    Objective: Erythrina variegata had been widely used for its reported biological activity in indigenous system of medicine. The present investigation was carried out to find the anti-inflammatory effect of ethanolic extract of Erythrina variegata in albino rats.Methods: The anti-inflammatory activity was evaluated using acute inflammatory model like carrageenan induced paw edema and chronic inflammatory model like cotton pellet induced granuloma respectively.Results: The ethanolic extract in different doses (200, and 400mg/kg, p. o) exhibited dose dependent and significant antiinflammatory activity in acute and chronic model of inflammation.Conclusion: The alcoholic extract of Erythrina variegata has anti-inflammatory activity. This activity was related to the dose and these results corroborate the potential traditional use of the plant in folk medicine

    Senyawa Antikanker Dari Dadap Ayam (Erythrina Variegata)

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    Dadap ayam (Erythrina variegata) secara tradisional dikenal oleh masyarakat lokal sebagai obat antikanker. Bagian tumbuhan ini yang biasa digunakan sebagai pengobatan adalah daun, tetapi kandungan senyawa kimia aktif biologisnya belum banyak dilaporkan. Pencarian komponen aktif yang terdapat di dalam tumbuhan E. variegata sangat penting sebagai nilai tambah untuk bahan obat herbal antikanker. Tujuan penelitian ini adalah untuk mengetahui senyawa yang beraktivitas antikanker dari bagian daun dan kulit batang E. variegata secara in vitro terhadap sel kanker payudara T47D. Penelitian dilakukan dengan cara ekstraksi metanol dan fraksionasi dari daun serta kulit batang E. variegata yang dipandu dengan uji antikanker secara in vitro menggunakan metode Sulforhodamin B (SRB). Selanjutnya, fraksi aktif dipisahkan komponen-komponennya dengan kombinasi kolom kromatografi yang dipandu dengan uji antikanker secara in vitro, diperoleh tiga senyawa aktif (1-3). Struktur kimia senyawa aktif (1-3) ditetapkan berdasarkan data spektroskopi dan perbandingan data dari senyawa yang berhubungan dari penelitian sebelumnya, dan diidentifikasikan sebagai turunan steroid (1-3). Senyawa (1-3) memperlihatkan aktivitas antikanker secara in vitro terhadap kanker payudara T47D dengan IC50 masing-masing 6,5; 5,3; dan 3,2 ?g/ml. Daun dan kulit batang E. variegata menunjukkan sumber bahan antikanker payudara

    Senyawa Aktif Antikanker Payudara Dan Antimalaria Dari Tumbuhan Dadap Ayam (Erhythrina Valeriegata) Secara in Vitro (Anti Breast-cancer and Anti-malarial Active Compounds of Erithrina Variegata by in Vitro Test)

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    Tumbuhan Erythrina variegata (Leguminosae) secara tradisional dikenal oleh masyarakat Indonesia sebagai obat antikanker dan antimalaria. Bagian tumbuhan ini yang biasa digunakan sebagai bahan pengobatan adalah daun dan kulit batang, tapi kandungan senyawa kimia aktif biologisnya belum banyak dilaporkan. Tujuan penelitian ini adalah untuk mengungkapkan senyawa aktif antikanker dan antimalaria secara in vitro yang terdapat di dalam daun dan kulit batang E. variegata. Penelitian dilakukan dengan cara ekstraksi metanol dan fraksionasi dari daun dan kulit batang E. variegata yang dipandu dengan uji antikanker secara in vitro terhadap sel kanker payudara T47D menggunakan metode Sulforodamin B (SRB) dan uji antimalaria secara in vitro terhadap Plasmodium falciparum 3D7 (sensitif klorokuin) dan K1 (resisten klorokuin) menggunakan metode laktatdehidrogenase (LDH). Selanjutnya dilakukan pemisahan fraksi etil asetat daun dan kulit batang E. variegata yang dipantau dengan uji antikanker dan antimalaria secara in vitro menggunakan kombinasi kolom kromatografi diperoleh tiga senyawa aktif (1, 2, dan 3). Struktur kimia senyawa aktif (1, 2, dan 3) ditetapkan berdasarkan data-data spektroskopi dan diidentifikasikan sebagai turunan triterpenoid pentasiklik glikosida(1); flavonoid, eristagallin A (2); dan steroid, (22E)-5α,8α-epidioksiergosta-6,22-dien-3ÎČ-ol (3). Senyawa (1) menunjukkan aktivitas antimalaria secara in vitro terhadap P. falciparum strain 3D7 (sensitif klorokuin) dengan IC50 1,8 ”g/mL dan terhadap strain K1 (resisten klorokuin) dengan IC50 3,3 ”g/mL. Senyawa (2) dan (3) menunjukkan aktivitas antikanker secara in vitro terhadap sel kanker payurada T47D dengan IC50 masing-masing 3,0 dan 3,2 ”g/mL. Tumbuhan E. variegata mempunyai potensi sebagai bahan dasar obat herbal antikanker payudara dan antimalaria

    IN VITRO EVALUATION OF ANTICANCER POTENTIAL OF ERYTHRINA VARIEGATA L. ON BREAST CANCER CELL LINES

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    Objective: Species of Erythrina variegata L. is reported to be used in the treatment of cancer in traditional/folklore medicine which could be explored for their anticancer potential. We aimed to evaluate the anticancer activity of crude extracts of the leaves of E. variegata with two solvents; explore the mechanism of cytotoxicity with the effective extract and correlation with the phytochemicals in the extract.Methods: The extracts with Erythrina variegata L methanol (EVM) and chloroform (EVC) as solvents were screened for cytotoxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay on MDA-MB-231 and MCF-7 cell lines. The effective extract was further evaluated on MDA-MB-231 cells by nucleoprotein content estimation, and cell morphology was studied. High resolution liquid chromatography mass spectrometry (HRLCMS) of EVM was done to find the phytochemical composition.Results: Among the two extracts, EVM was effective at an inhibitory concentration (IC50) value of 92 ĂŽÂŒg/ml and 143 ĂŽÂŒg/ml on MCF-7 and MDA-MB-231 cells, respectively. At the IC50 value (143 ĂŽÂŒg/ml) the nucleoprotein content of the cells was 58.2%, and the apoptotic index was calculated to be 51.8%. EVM treated group showed significant morphological changes suggestive of apoptosis. HRLCMS revealed the presence of rutin, podocarpatriene, and cepharanthine which are known to be cytotoxic.Conclusion: This report is a contribution toward the validation of E. variegata as a potential source of anticancer agents

    Potential of Dadap Ayam (Erythrina Variegata) Plant as Herbal Medicine

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    Traditional medicines originating from plants have been widely used as an alternative therapy and extensive research have identified several bioactive compounds of the plants. In Indonesia, Erythrina variegata (Leguminosae) plants have been used for medication to many diseases including anthelmintic, anticancer,antimalaria, and antifertility.Objective: In the course of our continuing search for novel bioactive compounds from Indonesian Erythrina plants, we isolated and described several bioactive compounds such as anticancer, antimalaria, and antifertility from E. variegata. Method: Activity assay in vitro against breast cancer cell T47D using the Sulforhodamine B (SRB) method, activity assay in vitro against antiplasmodium using lactatedehydrogenase (LDH) method, and antifertility activity on spermatozoa Rattus novergicus. Their chemical structures were determined based on spectroscopic evidences and comparison with related compound previously reported. Result: Extract and compounds of E. variegata showed anticancer in vitro against breast cancer cell T47D, antiplasmodial in vitro K1 and 3D7 strain parasites, and antifertility on spermatozoa R. norvegicus with midle and high activity. Besides that, the toxicity assay of it was also conducted using white mice to show the safety grade of the plant. Conclusion: This results strongly suggested that E. variegata is a promising sources as herbal medicine of anticancer, antimalaria, and antifertility agents
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