La mycothérapie au Burkina Faso : État des lieux et perspectives

Objectif : Les vertus médicinales des champignons supérieurs sont connues depuis des millénaires, notamment à travers la médecine asiatique. En Afrique de l’ouest, les recherches menées dans ce domaine sont rares. Cette étude est une contribution à la connaissance des champignons supérieurs utilisés dans la médecine traditionnelle au Burkina Faso. Méthodologie et résultats : L’étude a été réalisée dans la ville de Ouagadougou à partir d’enquêtes ethnomycologiques auprès de 60 tradipraticiens. Les résultats obtenus à l’issue de ces enquêtes ont permis d’identifier huit (8) espèces de champignons supérieurs utilisées en tradithérapie. Il s’agit de : Daldinia eschscholzii, Ganoderma lucidum, Ganoderma resinaceum, Phellinus pachyphloeus, Podaxis pistillaris, Lentinus squarrosulus, Lycoperdon sp et Scleroderma sp. L’analyse des données montre que le genre et l’âge des personnes interrogées n’ont pas d’influence sur la connaissance des espèces thérapeutiques. En revanche, les femmes connaissent plus d’espèces de champignons thérapeutiques que les hommes et la différence est significative entre les deux groupes. Conclusion et application des résultats : Ce premier inventaire non exhaustif des champignons thérapeutiques nous révèle une richesse insoupçonnée pour une région aride et considérée comme ayant une flore mycologique pauvre.L’étude a permis d’identifier huit espèces de champignons supérieurs médicinaux dans la ville de Ouagadougou au Burkina Faso. Ce sont : Daldinia eschscholzii, Ganoderma lucidum, Ganoderma resinaceum, Phellinus pachyphloeus, Podaxis pistillaris, Lentinus squarrosulus, Lycoperdon sp et Scleroderma sp. Il s’agit pour la plus part d’espèces lignicoles. Ces champignons supérieurs sont des agents bioactifs potentiels procurant des sources de médicaments aux populations burkinabés. Ces espèces pourraient être valorisées sur le plan thérapeutique. Leur identification constitue un préalable dans la gestion des ressources locales. Il serait aussi intéressant de mener des études plus approfondies afin de pouvoir mettre en évidence les substances bioactives en vue de leur vulgarisation.Mots clés : Mycothérapie, champignons, enquête ethnomycologique, Burkina FasoObjectives: The medicinal properties of mushrooms have been known for thousands of years, particularly through the Asian medicine. In West Africa and Burkina Faso specifically, research in this area is rare. The main objective of this study is to collect information on the use of mushrooms in traditional medicine in Burkina Faso. Methodology and results: This study was done in Ouagadougou. A quantitative questionnaire was used among 60 traditional healers (30 women and 30 men) in the central part of Burkina Faso to assess the knowledge of medicinal mushroom and how they are used. Eight mushroom species were identified in traditional medicine: Daldinia eschscholzii, Ganoderma lucidum, Ganoderma resinaceum, Phellinus pachyphloeus, Podaxis pistillaris, Lentinus squarrosulus, Lycoperdon sp and Scleroderma sp. The data analysis showed that the gender and the age of the informants have no influence on the knowledge of therapeutic species. Conclusion and application of results : This first nonexhaustive inventory of therapeutic mushrooms reveals us an unsuspected richness for an area arid and regarded as having a poor mycological flora This study have help to identify eight medicinal mushroom species in the town of Ouagadougou (Burkina Faso). This is: Daldinia eschscholzii, Ganoderma lucidum, Ganoderma resinaceum, Phellinus pachyphloeus, Podaxis pistillaris, Lentinus squarrosulus, Lycoperdon sp and Scleroderma sp. Most of them are lignicolous species.These higher mushrooms are potential bioactive agents getting sources of drugs to the burkinabé people. These species could be developed on the therapeutic level. Their identification constitutes a precondition in the local stock management.It would be also interesting to undertake studies in order to be able to highlight the bioactive substances for their popularization.Keywords: Mycotherapy, mushrooms, survey, Burkina Fas

The phylogenetic position of Rhopalostroma as inferred from a polythetic approach

The xylariaceous genus Rhopalostroma comprises a small conglomerate of stromatic, angiosperm-associated pyrenomycetes, which have so far exclusively been reported from the palaeotropics, above all from tropical Africa and South Asia. Morphological and chemotaxonomic studies had suggested their close relationship to the genera Daldinia and Hypoxylon. However, those results were mainly based on herbarium specimens, and no molecular phylogenetic data were available on Rhopalostroma. During a foray in Côte d’Ivoire, fresh material of R. angolense was collected, cultured and studied by microscopic methods and by secondary metabolite profiling using high performance liquid chromatography coupled with diode array and mass spectrometric detection. In addition, ITS nrDNA sequences of the cultures were generated and compared to those of representative Xylariaceae taxa, to evaluate the phylogenetic affinities of this fungus. The results showed that R. angolense is closely related to the daldinoid Xylariaceae, and in particular to the predominantly neotropical genera Phylacia and Thamnomyces

Unveiling the potential of Daldinia eschscholtzii MFLUCC 19-0629 through bioactivity and bioinformatics studies for enhanced sustainable agriculture production

Endophytic fungi constitute a rich source of secondary metabolites that can be manipulated to produce desirable novel analogs for combating current agricultural challenges for crop production, especially controlling plant disease. The endophytic fungus Daldinia eschscholtzii MFLUCC 19-0629, was newly isolated from tropical ancient plants, Oncosperma sp., and displays a broad-spectrum of antifungal and antibacterial activities against several plant pathogens including Ralstonia solanacearum, Fusarium oxysporum, Colletotrichum gloeosporioides, Colletotrichum acutatum, Stagonosporopsis cucurbitacearum, Corynespora cassiicola and Stemphylium spp. A high-quality genome sequence was obtained using Oxford nanopore technology, the accuracy and length of reads resulting in no need for Illumina or other sequencing techniques, for D. eschscholtzii MFLUCC 19-0629, resulting in a genome size of 37.56 Mb assembled over 11 contigs of significant size, likely to be at the chromosomal level. Bioinformatics analysis revealed that this strain is biosynthetically talented encoding 67 predicted biosynthetic gene clusters (BGCs). Only eight of the 67 BGCs matched or demonstrated high similarity to previously characterized BGCs linked to the production of known secondary metabolites. The high number of predicted unknown BGCs makes this strain a promising source of novel natural products. The discovery that D. eschscholtzii MFLUCC 19-0629 has a broad spectrum of antimicrobial activity against seven major plant pathogenic microorganisms relevant to crop production and its complete genome sequence carries immense importance in the advancement of novel microbial biocontrol agents (MBCAs). This also unveils the prospect of uncovering new compounds that could be utilized for sustainable agriculture and pharmaceutical purposes

Daldinia eschscholzii (Ascomycota, Xylariaceae) isolado na Amazônia brasileira: características taxonômicas e condições de crescimento micelial

The Amazon has a high diversity of fungi, including species of the genus Daldinia (Ascomycota, Xylariaceae), which produce secondary metabolites with recognized nematicidal and antimicrobial activity. The ecological role of Daldinia is important, as stromata serve as refuges to many insects and arthropodes, and the fungi contribute to the degradation of vegetable organic matter. The aim of this study was to analyze the taxonomic features and mycelial growth conditions in vitro of a Daldinia specimen collected in the Brazilian Amazon. Morphological and molecular studies of the fungus identified it as D. eschscholtzii. To evaluate mycelial growth, we cultivated the fungus at 20, 25, 30, 35, and 40 °C in malt extract-peptone agar (MEPA), malt extract-peptone (MEP), potato dextrose (PD), and minimum medium (MM). The best mycelial growth occurred at 35 °C, although the greatest amount of biomass was obtained at 25 °C and 30 °C. PD proved to be the best medium for biomass production.A Amazônia apresenta alta diversidade de fungos, incluindo Daldinia (Ascomycota, Xylariaceae), cujas espécies produzem metabólitos secundários com reconhecida atividade antimicrobiana e nematicida. O papel ecológico é importante, visto que estromas servem de abrigo para muitos insetos e artrópodes, além de contribuir na degradação da matéria orgânica vegetal. O objetivo desse estudo foi analizar as características taxonômicas e as condições do crescimento micelial in vitro de um espécime de Daldinia coletado na Amazônia brasileira. Estudos morfológicos e moleculares do fungo o indetificaram como D. eschscholtzii. Para avaliação do crescimento micelial o fungo foi cultivado nas temperaturas de 20, 25, 30, 35 e 40 °C e nos meios de cultura extrato de malte-peptona ágar (EMPA), extrato de malte-peptona (EMP), batata dextrose (BD) e meio mínimo (MM). O melhor crescimento micelial ocorreu a 35 °C, entretanto, a maior quantidade de biomassa foi obtida a 25 e 30 °C. O meio BD provou ser o melhor meio para produção de biomassa

The use of indol derivatives in medicine

Catedra de chimie farmaceutică și toxicologică, Universitatea de Stat de Medicină și Farmacie “Nicolae Testemițanu“ din Republica MoldovaIntroducere. Indolul este un compus organic heterociclic aromatic. Are o structură biciclică, formată din inelele benzenic și pirolic. Se găsește în abundență în uleiuri esențiale ale plantelor și poate fi considerat drept fondatorul unei clase noi de compuși atât naturali, cât și sintetici, care și-au găsit utilizare în medicină. Scopul lucrării. Generalizarea și prezentarea materialelor studiate anterior despre utilizarea derivaților indolului în medicină. Material și metode. Sinteza și analiza datelor bibliografice, selectate din baze de date internaţionale: PubMed, Medline, Environmental Issues & Policy Index, Google Academic etc. Rezultate. Analiza datelor din literatură demonstrează că indolul reprezintă unul dintre cele mai privelegiate structuri utilizate în sinteza și crearea de noi medicamente. Derivații de indol au proprietatea unică de a imita structura peptidelor și de a se lega reversibil de enzime, care oferă oportunități extraordinare de a descoperi medicamente noi cu diferite mecanisme de acțiune. Pe lângă substanțe deja cunoscute în medicină ( rezerpina, arbidol, vincamina, harmol), actualmente în terapie se utilizează și alte substanțe, cu un potențial promițător. Așadar, sumatriptan se administrează în tratamentul migrenei, iar etodolac și tenidap reprezintă o toxicitate mai mică decât indometacina. Concluzii. Derivații indolului au o largă aplicare în tratamentul celor mai diverse maladii, astfel scheletul indolic demonstrându-și aplicabilitatea și reactivitatea în sinteza de compuși cu perspectivă în medicină.Introduction. Indole is a heterocyclic aromatic, organic compound. It has a bicyclic structure, consisting of a benzen and a pyrolic ring. It is found in abundance in the essential oils of the plants and can be considered as a new class of comppounds founder of both natural and synthetic, that have found use in medicine. The aim of the study. Generalization and reporting of previously studied materials on the use of indole derivatives in medicine. Material and methods.Synthesis and analysis of bibliographic data, selected from international databases: PubMed, Medline, Environmental Issues & Policy Index, Google Scholar etc. Results. The analysis of the literature shows that indole is one of the most priveleged structures used in the synthesis and drug design. Indole derivatives have the unique property of mimicking the structure of peptides and reversible binding to enzymes, which offers extraordinary opportunities to discover new drugs with different mechanisms of action. In addition to substances already known in medicine( reserpine, arbdol, vincamine, harmol), other substances with a promising potential are currently being used in therapy. Therefore, sumatriptan is used in treatment of migraine, etodolac and tenidap are less toxi than indomethacin. Conclusions. Indole derivatives have a wide application in the treatment of various diseases, thus indole scaffold demonstrating its applicability and reactivity in the synthesis of compunds with medical perspective

Endophytic fungi of Tectona grandis L. (Teak).

Taxonomic diversity, biology and ecological aspects of fungal endophytes of Tectona grandis (teak) from Chiang Mai Province, Thailand were investigated. It was found that the endophyte assemblages of mature leaves sampled from natural forest and plantation teak were not significantly different. Members of the Xylariaceae, especially Daldinia eschscholzii, Nemania subannulata, Hypoxylon haematostroma and Xylaria cubensis were frequent isolates. Widely reported endophytic fungi such as Phomopsis, Colletotrichum, Cladosporium and Fusarium were also isolated. There is little evidence to support host specificity for the majority of the isolates. Differences in endophyte assemblages between young and mature leaves were shown to occur with a much lower infection percentage in the young leaves. Species of Phomopsis and Colletotrichum were dominant in the young leaves but members of the Xylariaceae dominated in mature leaves. This pattern was the same for both natural forest and plantation samples. However comparison of taxa isolated from leaf lamina, midrib and veins gave conflicting results. Samples from mature leaves from natural forest trees exhibited little variation with greater variation in taxa recovered being found to occur between sampling years than between position of isolation from the leaf. In plantation leaves, although the results were similar to those from natural forest tree leaves for two of the years sampled, in 1997 the overall recovery rate was highest for the lamina, followed by veins and then the midrib. There was no evidence obtained to link individual taxa with specific regions of the leaf. It is now possible to devise a sampling strategy to obtain suitable diversity of endophytic isolates from teak leaves for industrial screening of these fungi. Techniques were developed to overcome current problems of identification of xylariaceous endophytes in the absence of their teleomorph. Inoculation of suitable woody substrata combined with selective incubation was used to induce teleomorph formation in many of the isolates and this together with chemical profiling enabled identification to species of many of these isolates. Rates of development of specific species were obtained and differences in environmental conditions necessary for development of teleomorphs to maturity were noted for members of different genera. Thus species of Daldinia and Hypoxylon required drier conditions than species of Xylaria and Nemania which only developed under wet shaded conditions. Xylariaceae from the natural forest, plantation, and forest surrounding the plantation were surveyed and a number of the Xylariaceae recovered as endophytes were found to be new to science, new records for Thailand or were recorded as endophytes for the first time

Endophytic Fungi Associated With Turmeric (Curcuma longa L.) Can Inhibit Histamine-Forming Bacteria in Fish

Turmeric (Curcuma longa L.) is a medicinal plant that is commonly used as spice and preservative. Many types of endophytic fungi have been reported as being associated with medicinal plants and able to synthesize secondary metabolites. In this study, endophytic fungi were isolated from all plant parts of turmeric plants. Identification of the endophytic fungi was done using morphological characteristics and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA. The dual culture method was used for screening antibacterial activity of the endophytic fungi against Morganella morganii, a common histamine-producing bacteria. The disc diffusion method was used to test the ability of water fractions of selected endophytic fungi to inhibit M. morganii growth. Two-dimensional thin layer chromatography was used to determine the fungal extract inhibition activity on histamine formation. In total, 11 endophytic fungi were successfully isolated and identified as Arthrobotrys foliicola, Cochliobolus kusanoi, Daldinia eschscholzii, Fusariumoxysporum, Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Phanerochaete chrysosporium, and Phaeosphaeria ammophilae. Five isolates showed inhibition activity against M. morganii in the dual culture tests. Based on the disc diffusion assay, A. foliicola and F. verticillioides inhibited the growth of M. morganii as a histamine-producing bacteria, and inhibiting histamine formation in fish. The best effects in inhibiting growth of the histamine-producing bacteria and histamine formation inhibition in fish were produced with F. verticillioides water fraction at 0°C incubation

Biodiversidad de la familia Xylariaceae (Ascomycota) y sus hospedantes en áreas protegidas de Tucumán (Argentina)

Fourty nine species belonging to the genera Astrocystis, Biscogniauxia, Daldinia, Entonaema, Hypoxylon, Kretzschmaria, Phylacia, Poronia, Rosellinia, Stilbohypoxylon and Xylaria were recorded in protected areas from Tucuman. The species are grouped in probably endemic, cosmopolitan, neotropical and pantropical. The biodiversity of Xylariaceae in this province is compared with those from other countries. Twenty-three host taxa are registered, most of them were dicots. Cinnamomum porphyrium and Podocarpus parlatorei were the two native hosts more colonized.Se registran cuarenta y nueve especies pertenecientes a los géneros Astrocystis, Biscogniauxia, Daldinia, Entonaema, Hypoxylon, Kretzschmaria, Phylacia, Poronia, Rosellinia, Stilbohypoxylon y Xylaria. Las especies se agrupan, según su distribución, en endémica probablemente, cosmopolita, neotropical y pantropical. Se compara la biodiversidad de Xylariales de la provincia de Tucumán con la de otros países. Se identifican veintitrés hospedantes, la mayoría de ellos dicotiledóneas, siendo Cinnamomum porphyrium y Podocarpus parlatorei las especies nativas más colonizadas.Fil: Sir, Esteban Benjamin. Fund.Miguel Lillo. Dir.de Botanica. Laboratorio Criptogámico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hladki, Adriana Ines. Fund.Miguel Lillo. Dir.de Botanica. Laboratorio Criptogámico; ArgentinaFil: Romero, Andrea Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentin