CTL Bulletin- 2013

Occasional information sheet; dates included are: Jan. 7; Mar. 13; Apr. 1, 15; May 29; Jul. 10https://neiudc.neiu.edu/ctlbulletin/1004/thumbnail.jp

CTL Bulletin- 2009

Occasional information sheet; dates included are: Jan. 15; Feb 15; Mar. 1; Apr. 15; May 1; Oct. 15; Nov. 1, 15; Dec. 1https://neiudc.neiu.edu/ctlbulletin/1002/thumbnail.jp

CTL Bulletin- 2005

Occasional information sheet; dates included are: Jan. 15; Feb 15; Mar. 15; Apr. 15; Sept. 1, 15https://neiudc.neiu.edu/ctlbulletin/1000/thumbnail.jp

CTL Bulletin- 2006

Occasional information sheet; dates included are:Feb. 15; Mar. 15; Oct. 15https://neiudc.neiu.edu/ctlbulletin/1001/thumbnail.jp

CTL Bulletin- 2010

Occasional information sheet; dates included are: Jan. 15; Feb. 1; Mar. 15; May 1https://neiudc.neiu.edu/ctlbulletin/1003/thumbnail.jp

Frequency analysis of cytolytic T lymphocyte precursors (CTL-P) generated in vivo during lethal rabies infection of mice. II. Rabies virus genus specificity of CTL-P

Cytolytic T lymphocyte precursors (CTL-P) were sensitized in vivo by intraplantar infection of C57BL/6 mice with a lethal dose of rabies virus, strain ERA (ERA). As a result of sensitization CTL-P matured to interleukin-receptive CTL-P (IL-CTL-P) that could be expanded in vitro to Thy-1+, Lyt-2+ CTL clones in the presence of IL without subjection to antigen-driven selection. After infection with ERA, IL-CTL-P-derived CTL lysed fibroblasts infected with rabies virus but not those infected with another rhabdovirus, the vesicular stomatitis virus. These CTL, however, did not discriminate between fibroblasts infected with the serologically closely related laboratory strains of classic rabies virus, ERA and HEP-Flury, and the serologically distinct rabies-related African isolate Mokola. This finding implies that in vivo sensitized IL-CTL-P recognize common genus-specific determinants expressed on cells infected with members of the lyssavirus genus

On the Hybrid Extension of CTL and CTL+

The paper studies the expressivity, relative succinctness and complexity of satisfiability for hybrid extensions of the branching-time logics CTL and CTL+ by variables. Previous complexity results show that only fragments with one variable do have elementary complexity. It is shown that H1CTL+ and H1CTL, the hybrid extensions with one variable of CTL+ and CTL, respectively, are expressively equivalent but H1CTL+ is exponentially more succinct than H1CTL. On the other hand, HCTL+, the hybrid extension of CTL with arbitrarily many variables does not capture CTL*, as it even cannot express the simple CTL* property EGFp. The satisfiability problem for H1CTL+ is complete for triply exponential time, this remains true for quite weak fragments and quite strong extensions of the logic

On the Complexity of ATL and ATL* Module Checking

Module checking has been introduced in late 1990s to verify open systems, i.e., systems whose behavior depends on the continuous interaction with the environment. Classically, module checking has been investigated with respect to specifications given as CTL and CTL* formulas. Recently, it has been shown that CTL (resp., CTL*) module checking offers a distinctly different perspective from the better-known problem of ATL (resp., ATL*) model checking. In particular, ATL (resp., ATL*) module checking strictly enhances the expressiveness of both CTL (resp., CTL*) module checking and ATL (resp. ATL*) model checking. In this paper, we provide asymptotically optimal bounds on the computational cost of module checking against ATL and ATL*, whose upper bounds are based on an automata-theoretic approach. We show that module-checking for ATL is EXPTIME-complete, which is the same complexity of module checking against CTL. On the other hand, ATL* module checking turns out to be 3EXPTIME-complete, hence exponentially harder than CTL* module checking.Comment: In Proceedings GandALF 2017, arXiv:1709.0176

Frequency analysis of cytolytic T cell precursors (CTL-P) generated in vivo during lethal rabies infection of mice. I. Distinction of CTL-P with different interleukin 2 sensitivity

The aim of this study was to determine the number and state of activity of cytolytic T lymphocytes (CTL) and their precursors (CTL-P) present in vivo during the early stages of viral infection. The local response to lethal infection with rabies virus was used as a model system that is not accessible to analysis by secondary activation in vitro. The local response to alloantigen served as a control. Experimental protocols were established that allow frequency estimates of in vivo antigen-triggered CTL-P. Data allow a distinction between CTL-P activated in vivo by alloantigen and viral antigen with respect to their different capacity to utilize T cell growth factors (inter-leukins). In vivo alloantigen-primed CTL-P generate, in vitro, an active effector progeny in the presence of interleukins of xenogeneic origin, whereas the majority of virus-specific CTL-P, in spite of considerable expansion in vivo, fail to generate CTL in vitro unless antigen is added