Lewis X antigen mediates adhesion of human breast carcinoma cells to activated endothelium. Possible involvement of the endothelial scavenger receptor C-Type lectin

Lewis x (Lex, CD15), also known as SSEA-1 (stage specific embryonic antigen-1), is a trisaccharide with the structure Galβ(1–4)Fucα(1–3)GlcNAc, which is expressed on glycoconjugates in human polymorphonuclear granulocytes and various tumors such as colon and breast carcinoma. We have investigated the role of Lex in the adhesion of MCF-7 human breast cancer cells and PMN to human umbilical endothelial cells (HUVEC) and the effects of two different anti-Lex mAbs (FC-2.15 and MCS-1) on this adhesion. We also analyzed the cytolysis of Lex+-cells induced by anti-Lex mAbs and complement when cells were adhered to the endothelium, and the effect of these antibodies on HUVEC. The results indicate that MCF-7 cells can bind to HUVEC, and that MCS-1 but not FC-2.15 mAb inhibit this interaction. Both mAbs can efficiently lyse MCF-7 cells bound to HUVEC in the presence of complement without damaging endothelial cells. We also found a Lex-dependent PMN interaction with HUVEC. Although both anti-Lex mAbs lysed PMN in suspension and adhered to HUVEC, PMN aggregation was only induced by mAb FC-2.15. Blotting studies revealed that the endothelial scavenger receptor C-type lectin (SRCL), which binds Lex-trisaccharide, interacts with specific glycoproteins of Mr␣∼␣28 kD and 10 kD from MCF-7 cells. The interaction between Lex+-cancer cells and vascular endothelium is a potential target for cancer treatment.Fil: Elola, Maria Teresa. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capurro, Mariana Isabel. University of Toronto; CanadáFil: Barrio, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación, Docencia y Prevención del Cáncer; ArgentinaFil: Coombs, Peter J.. Imperial College London; Reino UnidoFil: Taylor, Maureen E.. Imperial College London; Reino UnidoFil: Drickamer, Kurt. Imperial College London; Reino UnidoFil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación, Docencia y Prevención del Cáncer; Argentin

Searching for BSM neutrino interactions in dark matter detectors

Neutrino interactions beyond the Standard Model (BSM) are theoretically well motivated and have an important impact on the future precision measurement of neutrino oscillation. In this work, we study the sensitivity of a multi-ton-scale liquid Xenon dark matter detector equipped with an intense radiative neutrino source to various BSM neutrino-electron interactions. We consider the conventional Non-Standard Interactions (NSIs), other more generalized four-fermion interactions including scalar and tensor forms, and light-boson mediated interactions. The work shows that with realistic experimental setups, one can achieve unprecedented sensitivity to these BSM neutrino-electron interactions.Comment: fig. 7 added, matches the published versio

Cosmic ray energy changes at the termination shock and in the heliosheath

Voyager 1 crossed the termination shock of the solar wind in December 2004 at 94 AU and currently measures the cosmic ray intensity in the heliosheath. To better understand this modulation region beyond the shock, where adiabatic energy changes should be small, we review the net effect of energy changes during the modulation process, including adiabatic deceleration in the solar wind, acceleration at the termination shock, and the possibility that stochastic acceleration in the heliosheath may also make a contribution

Cosmic-Ray Spectra in Interstellar Space

At energies below ~300 MeV/nuc our knowledge of cosmic-ray spectra outside the heliosphere is obscured by the energy loss that cosmic rays experience during transport through the heliosphere into the inner solar system. This paper compares measurements of secondary electron-capture isotope abundances and cosmic-ray spectra from ACE with a simple model of interstellar propagation and solar modulation in order to place limits on the range of interstellar spectra that are compatible with both sets of data

Probing Active to Sterile Neutrino Oscillations in the LENS Detector

Sterile neutrino conversion in meter scale baselines can be sensitively probed using monoenergetic, sub-MeV, flavor pure e-neutrinos from an artificial MCi source and the unique technology of LENS designed to oberve the low energy solar neutrino spectrum via tagged CC e-neutrino capture in 115-In. Active-sterile oscillations can be directly observed in the granular LENS detector itself to critically test and extend resuls of short baseline accelerator and reactor experiments.Comment: 4pages, 4 figures, text and figure change

Revised neutrino-gallium cross section and prospects of BEST in resolving the Gallium anomaly

O(1) eV sterile neutrino can be responsible for a number of anomalous results of neutrino oscillation experiments. This hypothesis may be tested at short base-line neutrino oscillation experiments, several of which are either ongoing or under construction. Here we concentrate on the so-called Gallium anomaly, found by SAGE and GALLEX experiments, and its foreseeable future tests with BEST experiment at Baksan Neutrino Observatory. We start with a revision of the neutrino-gallium cross section, that is performed by utilizing the recent measurements of the nuclear final state spectra. We accordingly correct the parameters of Gallium anomaly and refine the BEST prospects in testing it and searching for sterile neutrinos. We further evolve the previously proposed idea to investigate the anomaly with Zn-65 artificial neutrino source as a next option available at BEST, and estimate its sensitivity to the sterile neutrino model parameters following the Bayesian approach. We show that after the two stages of operation BEST will make 5$\sigma$-discovery of the sterile neutrinos, if they are behind the Gallium anomaly.Comment: 7 pages, 8 figures; v2: 8 pages, 9 figures, journal versio