A high-density high-yield fat rate oil of microalgae culture

Abstract

本发明属于单细胞油脂及生物柴油生产技术领域,特别涉及一种同步式提高产油微藻的生物量和产脂量的方法。取对数生长期的产油微藻,培养至添加有碳源和/或氮源的无菌添加液的液体培养基中,在温度为18-35℃,转速为100-200rpm,光照强度为0-150μmol?m-2s-1连续光照的摇床中振荡培养6-40天。其中,添加有碳源和/或氮源的添加液的液体培养基中培养过程中再次添加无菌添加液。本发明将产油微藻的生长过程和产脂过程通过交替、循环的控制模式进行技术集成,既能提高藻细胞浓度,同时又能高效产脂,一举两得。无需更换培养基组合物,无需全营养养料分批供应,无需实时计算调整养料流加速率,不但操作简单,而且大大地降低了生产成本

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Institutional Repository of Yantai Institute of Coastal Zone Research, CAS

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Last time updated on 04/07/2017

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