Chemical composition, antiradical and antimicrobial effect assessment of Meadowsweet (Filipendula ulmaria L.) herbal raw material

Abstract

Objective: To assess chemical composition and biological effects of meadosweet (Filipendula ulmaria L.) Objectives: To determine the most suitable extraction method and the optimum extraction conditions; evaluation of quantitative composition dynamics of flavonoids, tannins and ascorbic acid during the growing season; antimicrobial effects evaluation of herbal ingredients against nine microbial cultures; evaluate dependence antiradical activity on the growing stage of the plant and to determine whether there is a relationship between quantity of flavonoids, tannins and ascorbic acid in the herbal raw material and its antiradical effect. The maceration by ultrasonic bath using 70 %. V / V ethanol was selected for extraction . For total flavonoids determination the spectrophotometric method was applied for total flavonoid content. For total tannin content and content of ascorbic the pharmacopoeial titration methods were applied. The antimicrobial effects were evaluated by infusing different concentrations of extractives to agar and observing microorganisms growth. The antiradical activity determined using the ABTS radical scavaning method. The highest total flavonoid content was found in massive flowering period. In the first half of the phenological development ascorbic acid content was higher than the average of the entire period of growing (> 0.025 %.). The peak of total tannin content was reached before massive flowering stage of meadowsweet (16.80 %). The ethanol extractives of meadowsweet were characterized by a slightly higher antimicrobial activity than the aqueous extractives, especially against E. faecalis and B. subtilis. The amount of quenched free radicals from the beginning of the growing to the massive flowering stage increased steadily and reached its peak at massive flowering of meadowsweet (95.33 ± 0.16 %.). The average correlation was found between total flavonoid content and the antiradical activity