DataSheet_1_Mendelian randomization reveals the impact of diet on infertility in men and women.docx

Abstract

BackgroundAlthough studies on the effects of diet on fertility has progressed, some cumulative evidence has piled against popular hypotheses. The aim of our study was to investigate the effects of 31 diets including 23 individual dietary intakes and 8 dietary habits on infertility in men and women.MethodsThe datas of diets and infertility were collected from genome-wide association studies (GWAS). Mendelian randomization (MR) methods were used to analyze causal relationships. Multivariate MR (MVMR) adjusted for the effects of other exposures on causality. And MR-Egger, Cochran’s Q, radial MR, and MR-PRESSO tests were employed to assess heterogeneity and horizontal pleiotropy.ResultsOur study found that coffee intake (OR, 3.6967; 95% CI, 1.0348 – 13.2065; P = 0.0442) and cooked vegetable intakes (OR, 54.7865; 95% CI, 2.9011 – 1030.5500; P = 0.0076) increased the risk of male infertility. For women, beer was a risk factor for infertility (OR, 4.0932; 95% CI, 1.8728 – 8.9461; P = 0.0004); but processed meat was negatively associated with infertility (OR, 0.5148; 95% CI, 0.2730 – 0.9705; P = 0.0401). MVMR demonstrated selenium as a protective factor against female infertility (OR, 7.4474e-12; 95% CI, 5.4780e-22 – 1.0125e-01; P = 0.0314).ConclusionWe found the causal relationships between four diets and infertility. We look forward to more high-quality epidemiologic studies to prove our conclusions.</p