Zebrafish electroretinogram responses


The goal of this project is to be able to streamline a protocol for conducting ERGs on zebrafish from mice ERG protocol already present in Dr. Renna’s lab. The objective is to create a protocol specifically for zebrafish and to eliminate any issues that occur when transiting from a mice ERG protocol to a zebrafish ERG protocol and to obtain a light response from zebrafish retinae in differing light intensities. Dr. Renna’s lab has designed an Ex Vivo ERG system with less electrical interference creating defined electrical responses from mouse retina. The setup allows for continual perfusion of the retinal tissue with chemical blockers to isolate specific components of the light response. The ERG is a commonly used method to investigate the visual system in both clinical and scientific settings to study the neurotransmission of the retina. Zebrafish are quickly becoming a good model organism for studying the visual system due to their utilization of cone dominated vision and their similar spectral sensitives to humans. I will test the hypothesis that an Ex Vivo ERG can be conducted on zebrafish retinae, a light response can be obtained, and a concise protocol can be created for zebrafish ERGs

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This paper was published in The University of Akron.

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