Josip Juraj Strossmayer University of Osijek. Department of biology.
Abstract
Biofilm je skup mikrobnih kolonija zatvorenih unutar samo-proizvedenog
ekstracelularnog polisaharidnog matriksa (glikokaliksa). U ovom je radu istražen te opisan utjecaj pesticida
na aktivnost biofilmova. Biofilmovi su razvijani u mikrotitracijskim pločicama gdje su kvantificirani pomoću
Kristal violet testa. Enzimska aktivnost je izmjerena pomoću UV-Vis spektrofotometra. Dokazano je da
manje koncentracije pesticida imaju slabiji utjecaj na razvoj biofilmova.Biofilm is an aggregate of microbial colonies enclosed in self-produced extracellular polysaccharide matrix
(glycocalyx). This paper researches and describes the effects of several types of pesticides on sole activity of
biofilms formed in the soil. Biofilms were developed in microtitle plates where they were quanitified using
Crystal violet assay. Enzym activitiy was measured using UV-Vis spectrophotomete. It was proven that
lower concentration of pesticide have weaker effect on the development of biofilms
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