Activation of human NK92 cells.

Abstract

<p>(a) IFN-γ ELISA. Cells of Lent-clones and Hela cells in triplicates were co-cultured with human NK92 cells and the supernatants were collected. IFN-γ activity in the supernatants was detected using Human IFN-γ ELISA Kit. The statistical analyses were conducted between the control (Hela+NK) and each Lent-clone (as the bars represented in the figure) using one-way analysis of variance (ANOVA) with Tukey’s post test. (*p<0.05; **p<0.01) (b) Cytotoxicity of NK92 cells. Cells of each lent-clone and Hela in triplicates were co-cultured with human NK92 cells. After co-culture, NK92 cells were removed and remaining tumor cells’ proliferation was measured with Promega’s CellTiter 96ueous nonRadioactive cell proliferation assay kit. The statistical analyses were conducted between the control (Hela+NK) and each virally transduced lent-clone using one-way analysis of variance (ANOVA) with the Tukey’s post test (*p<0.05; **p<0.01).</p