Sensitivity analysis of XMRV PCR assays.

Abstract

<p>PCR products were analyzed on agarose gels containing ethidium bromide. (A) Non-nested PCR assays targeting the XMRV <i>env</i> gene (top panel) and the <i>gag</i> gene (bottom panel), and (B) a nested PCR assay targeting the XMRV <i>env</i> gene were evaluated for their ability to detect either (A) provirus in XMRV-infected PNT1A cell DNA or (B) provirus in XMRV-infected LNCaP cell DNA diluted in uninfected cell DNA. Dilutions of infected cells in uninfected cells are indicated by ratios, i.e. 1∶10⁴ indicates one infected cell diluted in 10⁴ uninfected cells. (m) 100 base pair molecular weight marker, (H₂O) water used in place of DNA template as a negative control, (u) uninfected PNT1A DNA used as template for negative control.</p