Screen of a kinase inhibitor library for PKD1 activity.

Abstract

<p>A targeted library of 235 compounds was screened for PKD1 activity at 1 µM using an <i>in vitro</i> radiometric PKD1 kinase assay. The representative graphs show % residual PKD1 kinase activity calculated based on the total kinase activity measured in the absence of inhibitors (DMSO). Kb-NB142-70, a previously known PKD inhibitor, was used as a positive control. Experiments were performed with triplicate determinations at 1 µM for each compound.</p