Protocols for cerebral ischemia without and with postconditioning.

Abstract

<p>Animals were divided into three groups. All animals were subjected to MCA electrocoagulation, and bilateral CCA were transiently (50 min) occluded 2 min later. Re-flow was initiated by CCA occlusion release in controls (n = 17 in the first set, n = 11 in the second set and n = 11 in the third set of experiments). Postconditioning with 3 cycles (occlusion/reperfusion) of 30 s (n = 11), 1 (n = 18) or 5 (n = 12) min was performed on both or only left CCA within 15 s after the initial CCA reperfusion. Alternative reperfusion was initiated by first occlusion release of the left (L/R, n = 11) or of the right (R/L, n = 12) CCA followed by occlusion release of the other CCA.</p