Functional assessment of FRDA-3′-UTR versus WT-3′-UTR.

Abstract

<p> U2OS (black bars) and HEK-293 (grey bars) cells were transfected with luciferase reporter gene system, respectively 150 ng of empty plasmid or plasmid WT-3′-UTR or plasmid FRDA-3′-UTR. Histograms show the <i>Renilla</i> luciferase activity (normalized to firefly luciferase and to the mock transfected cells) following transfection of each plasmid into both cell lines. All results represent mean ± SEM of three independent experiments, each in triplicate. *<i>P</i><0.05, Student-t test.</p