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<p>Antigen specific IgG1 and IgG2a or IgG2b levels were determined using an IgG isotyping ELISA, as described in Materials and Methods. Sera were collected 2 weeks after the last immunization and diluted 1∶100. Bars indicate the average OD value at 450 nm (OD<sub>450</sub>) of each group.</p
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