Erythrocyte-binding activity of native PfRH5.

Abstract

<p>A Binding of the native PfRH5 protein in the HB3 culture supernatant incubated with untreated (WT) erythrocytes, variously enzyme-treated erythrocytes (N: neuraminidase; HT, high trypsin; C, chymotrypsin). The PfRH5 parasite protein was detected in the eluate fractions by immunoprecipitation with anti-RH5 antibodies in WT, N and C lanes but not HT. B The same eluate samples from (A) were used for the detection of EBA-175 by immunoprecipitation. EBA-175 binds to wild type and chymotrypsin-treated erythrocytes but not to neuraminidase and high trypsin-treated erythrocytes C Binding of the native RH5 protein to erythrocytes treated with lower concentrations of trypsin (LT low trypsin and MT: moderate trypsin). D The eluate samples from (C) were used for the detection of EBA-175 by immunoprecipitation. EBA-175 binds to untreated erythrocytes but not to low-trypsin- and moderate-trypsin-treated erythrocytes.</p