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<p>B7KO, CD28KO and WT C57BL/6 mice were sacrificed at 8-week of age. Total lymphocytes (left column) from viable cells, CD4<sup>−</sup>CD8<sup>−</sup> DN lymphocytes (middle column) and CD4<sup>+</sup> lymphocytes (right column) were analyzed for the expression of TCRβ and NK1.1. One representative profile from each group is presented. The numbers in the panels are percentages (Mean±SD) of gated NKT cells (n = 9). The numbers shown on the side of figures are <i>p</i> value between two indicated groups.</p
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