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oaioai:figshare.com:article/661224

A brief schematic of the assays for testing the effect of polyanions on HIV-1 infection in the presence of PAP248-286 or SE-F.

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Publication date
2013
Publisher
Doi

    Abstract

    <p>Polyanions at graded concentration was mixed with an indicated concentration of PAP248-286 or SE-F at an indicated interval with agitation as described in the “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059777#s2" target="_blank">Materials and Methods</a>”. After centrifugation, the supernatants were collected for testing the inhibitory activity of the unbounded polyanions on HIV-1 infection. The pellets containing the PAP248-286 (SEVI) - or SE-F-derived amyloid fibrils were re-suspended in 100 µl medium for testing the enhancing effect on HIV-1 infection.</p

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    The Francis Crick Institute

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    Last time updated on 12/02/2018

    This paper was published in The Francis Crick Institute.

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