HaeIII-digested terminal restriction fragments of 16S rRNA genes, which correlated with the sediment chemical parameters (Figure 3B) of the Baltic Sea and their identification.

Abstract

<p>T-RF = terminal restriction fragment, bp = base pairs.</p>a<p>Expected T-RFs based on virtual digestion of partial (appr. 400−500 bp) 16S rRNA gene sequences.</p>b<p>Observed T-RFs (27−700 bp) produced by terminal restriction fragment length polymorphism analysis of (1) 16S rRNA gene clones and (2) 16S rRNA genes of sediment samples.</p>c<p>Shift of 10 bp between expected and observed T-RFs was due to conditions in some of the capillary gel electrophoresis runs of digested 16S rRNA gene clones. Therefore, assignments of all T-RFs with observed lengths of 27−31 bp were used to identify T-RF 30 bp in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067061#pone-0067061-g003" target="_blank">Figure 3B</a>, derived from the sediment samples.</p>d<p><i>Bacteroidetes</i> incertae sedis.</p