Investigation of the voltage-dependence of PorACj in a multi-channel experiment.

Abstract

<p>The purified protein was added to the <i>cis</i>-side of a PC membrane (100 ng/ml) and the reconstitution of channels was followed until equilibrium. Then increasing positive (upper traces) and negative voltages (lower traces) were applied to the <i>cis</i>-side of the membrane, and the membrane current was measured as a function of time. The aqueous phase contained 1 M KCl; T = 20°C.</p