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<p><i>E. coli</i> JM109/pPT-G-CSF cells were grown in fed-batch culture using temperature shift method in a 300-L fermentor with glucose as the energy source. Optical density was detected using a spectrophotometer at 600 nm. Glucose concentration (grey triangle); OD<sub>600</sub> (β); Expression rate of rhG-CSF (β‘). The arrow indicates the start time of feeding.</p
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