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Cell growth and rhG-CSF expression during the fed-batch culture.

Abstract

<p><i>E. coli</i> JM109/pPT-G-CSF cells were grown in fed-batch culture using temperature shift method in a 300-L fermentor with glucose as the energy source. Optical density was detected using a spectrophotometer at 600 nm. Glucose concentration (grey triangle); OD<sub>600</sub> (●); Expression rate of rhG-CSF (β–‘). The arrow indicates the start time of feeding.</p

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The Francis Crick Institute

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Last time updated on 12/02/2018

This paper was published in The Francis Crick Institute.

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