Repository landing page

We are not able to resolve this OAI Identifier to the repository landing page. If you are the repository manager for this record, please head to the Dashboard and adjust the settings.

The <i>pil1</i> locus of <i>S. gallolyticus</i> strain UCN34.


<p>(A) Schematic representation of the <i>pil1</i> operon constituted of three genes: <i>pilA</i>, encoding the pilus adhesin, <i>pilB</i> encoding the major pilin and <i>srtC</i>, encoding the sortase required for pilus polymerization. Relevant domains are: COL, collagen-binding domain (Pfam 05737); and LPXTG, cell wall-anchoring domain. The P<i>pil1</i> promoter, the premature terminator upstream <i>pilA</i> and the putative terminator (hairpin structures) downstream <i>srtC</i> delineating the <i>pil1</i> operon are indicated. (B) Schematic representation of the intergenic region <i>gallo2180-pilA</i> with the 22 GCAGA repeats striped in gray and white and the stem-loop structure. (C) Determination of transcription start sites (TSS) of the <i>pil1</i> operon by primer extension analysis. The band indicated as “+1” corresponds to the location upstream the leader peptide gene is the likely TSS of the <i>pil1</i> operon; the band marked as “*” is located within one inverted repeat of the stem-loop structure and is likely generated by a pause of the RNA polymerase during elongation. (D) Nucleotide sequence of the intergenic region <i>gallo2180-pilA</i> showing the -35 and -10 promoter boxes and the +1 site, the leader peptide coding sequence with the 22 GCAGA repeats, the downstream stem-loop structure, the <i>pilA</i> initiator codon and the predicted ribosome binding site (RBS). (E) The predicted secondary structure of the stem-loop transcription terminator identified upstream ATG<i><sub>pilA</sub></i> (according to The mfold WebServer, <a href="" target="_blank"></a>).</p

Similar works

Full text



Last time updated on 12/02/2018

This paper was published in FigShare.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.