TIPE2 deficiency increases NO production but decreases urea production in macropahges.

Abstract

<p>Peritoneal macrophages from WT and <i>TIPE2^−/−</i> mice were treated with 100 ng/mL LPS for 0 h, 3 h, and 24 h. iNOS mRNA (<b>A</b>) and protein (<b>B</b>) levels were determined by quantitative PCR and Western blot, respectively. Expression levels of arginase I and arginase II mRNA were examined by quantitative RT-PCR (<b>D</b> and <b>C</b>). Cells were stimulated with 100 ng/mL LPS for 24 h, and culture supernatants were harvested for measurement of NO and urea (<b>E</b> and <b>F</b>). Data are shown as means ±SE (n = 4) of one representative experiment. *<i>P</i><0.05.</p