Histological evaluations of ovaries in treated, untreated control, and normal control animals by midline section and HE staining.

Abstract

<p>Ovary sections from sterilized non-transplanted mice after (A) a 7-day recovery period and (B) a 14-day recovery period, which showing the hollow structure destroyed by chemotherapy. Ovary sections from sterilized non-transplanted mice showing fibrosis and reduced hollow structure after a (C) 28-day recovery period and (D) 2-month recovery period. Ovary sections from sterilized recipient mice at (E) 7 days, (F) 14 days, (G) 28 days, and (H) 2 months after transplantation of SMSCs. Primordial follicles are visible in F (arrows), developing follicles at various stages are visible in G and H. However, there were fewer such developing follicles in G than in H. The morphology of ovary section of normal (I), untreated (J), and treated (K) control animals. (H) The changes in the ovarian volumes in treated, untreated control, and normal control (**<i>P</i><0.01, *<i>P</i><0.05). Scale bars: (F) 100 µm, (A–E, G–H) 200 µm, (I–K) 500 µm.</p