Simultaneous deletion of <i>cre-1</i> and <i>col-26</i> rescues the phenotype of Δ<i>vib-1</i> on cellulose.

Abstract

<p>(A) Cellulase activity of culture supernatants after 4-days of growth on Avicel from WT versus Δ<i>vib-1</i>, Δ<i>cre-1</i>, Δ<i>col-26</i>, Δ<i>cre-1</i>; Δ<i>vib-1</i>, Δ<i>col-26</i>; Δ<i>vib-1</i> and Δ<i>cre-1</i>; Δ<i>col-26</i>; Δ<i>vib-1</i> strains. (B) RT-PCR measurements of <i>clr-2</i> and <i>cbh-1</i> expression in the WT versus the Δ<i>col-26</i>, Δ<i>cre-1</i>, and the Δ<i>cre-1</i>; Δ<i>col-26</i>; Δ<i>vib-1</i> cultures after 5-days of growth on Avicel. Expression levels were normalized to WT. (C) The CMCase activity of Avicel cultures of WT versus Δ<i>col-26</i>, Δ<i>cre-1</i>, and the Δ<i>cre-1</i>; Δ<i>col-26</i>; Δ<i>vib-1</i> mutants during a time course of growth on Avicel.</p