Detection of <i>S. invicta</i> sNPF receptor in cell membranes prepared from the <i>Si</i>sNPFR-C6E8 cell line by western blot analysis.

Abstract

<p>Numbers in the left indicate the marker’s protein mass (kDa). Lane 1, the membrane fraction of the fire ant mated queen ovary (Ov) as a positive control; lane 2, the membrane fraction of <i>Si</i>sNPFR-C6E8 (C6E8) and lane 3, the membrane fraction of the vector-only (VO) (pcDNA3.1(-)) transfected CHO-K1 cells probed with the specific anti-sNPF receptor anti-peptide antibody <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109590#pone.0109590-Lu1" target="_blank">[44]</a>. Membrane protein (50 µg) was loaded in each lane. The antibody detects the 55 kDa receptor protein in lanes 1 and 2, as expected.</p