Gastrin-induced activation of SIK1.

Abstract

<p><b>A:</b> AR42J cells were treated with gastrin and mRNA levels measured by qRT-PCR. Mean expression level relative to untreated cells is shown. Results show one representative of three independent biological experiments; mean ± SD of three technical replicates. <b>B:</b> SIK1 Western blot of gastrin treated AR42J cells. A representative image is shown and quantified <b>C:</b> AGS-G_R cells were treated with gastrin and mRNA levels measured by qRT-PCR. Mean ± SEM of three independent biological experiments is shown. <b>D:</b> SIK1 Western blot of gastrin treated AGS-G_R cells. A representative image is shown and the SIK1 bands from two independent experiments were quantified; results shown are mean intensities ±SD. <b>E:</b> SIK1 Western blot of gastrin treated MKN45 cells. The SIK1 bands from a representative experiment were quantified. <b>F:</b> Intracellular localization of endogenous CRTC2 protein (Red; CRTC2, blue; Draq-5-stained DNA). G: Intracellular localization of SIK1 protein. AGS-G_R cells transfected with pEGFP-SIK1.</p