TJ-84 suppressed 5-FU-induced ROS production in mitochondria.

Abstract

<p>(A), Sa3 cells were incubated with or without 5 mg/mL of 5-FU for 6 h following a 1-h pre-incubation with 500 µg/mL of TJ-84. MitoSOX Red (5 µM) and 100 µM Mitotracker Green were then loaded for 30 min. ROS (red) and mitochondria (green) were detected by fluorescence microscopy. (B), The red fluorescence intensity per cell was calculated using ImageJ and is shown on the graph. Values are means ± S.E.M. (n = 63, 71, 75). **<i>p</i><0.01 compared to the control cells.</p