Inhibition of NLRP3 inflammasomes decreased 5-FU-induced cell death.

Abstract

<p>(A), Cell viability of Sa3 cells incubated with 5 mg/mL of 5-FU for 24 h after a 30-min pre-incubation with caspase inhibitor at each concentration. Values are means ± S.E.M. (n = 4). **<i>p</i><0.01 compared to the control group. (B), Expression of NLRP3 mRNA in Sa3 cells treated with NLRP3 siRNA (NLRP3) or scrambled oligo (scr). Values are means ± S.E.M. (n = 4). *<i>p</i><0.05 compared to cells without oligo (–). (C), Cell viability of cells transduced without (–) or with NLRP3 siRNA (NLRP3) or control oligo (scr) following an incubation with (closed bar) or without (open bar) 5 mg/mL of 5-FU for 3 h. Data are given as percentages compared to the group that was not incubated with 5-FU. Values are means ± S.E.M. (n = 6). *<i>p</i><0.05 compared to the control group. (D), Effect of transfection with NLRP3 siRNA (NLRP3) or scrambled oligo (scr) on 5-FU-induced LDH release. The LDH levels in the supernatants are given as percentages of cells not incubated with 5-FU and not transduced with siRNA. Values are means ± S.E.M. (n = 8). *<i>p</i><0.05 compared to the control cells.</p