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Effect of charges in wildtype and mutant HlyA on the single-channel conductance.

Abstract

<p>The single-channel conductance of the HlyA, HlyA<sub>Δ71–110</sub>, and HlyA<sub>Δ264–286</sub> channels is shown as a function of the KCl concentration in the aqueous phase. The solid lines represent the fit of the single-channel conductance data with eqn. <i>G(c) = G<sub>0</sub>⋅c<sub>0</sub><sup>+</sup></i> (a combination of eqs. (3–5 and 7) assuming the presence of negative point charges within the channel (for HlyA: 2.3 negative charges, <i>q = </i>−3.68×10<sup>–19</sup> As; for HlyA<sub>Δ71–110</sub>∶1.7 negative charges, <i>q</i> = -2.72×10<sup>–19</sup> As; for HlyA<sub>Δ264–286</sub>∶2 negative charges, <i>q = </i>-3.2×10<sup>–19</sup> As) and assuming a channel diameter of 2 nm, 1.4 nm, and 1.6 nm for HlyA, HlyA<sub>Δ71–110</sub>, and HlyA<sub>Δ264–286</sub>, respectively. <i>c</i>, concentration of the KCl solution in M (molar); <i>G</i>, average single-channel conductance in nS (nano Siemens, 10<sup>–9</sup> S); <i>G<sub>0</sub></i>, specific single-channel conductance in the absence of negative point charges given in pS/M. The broken, dotted, and fractured (straight) lines show the single-channel conductance of the HlyA, HlyA<sub>Δ264–286</sub>, and HlyA<sub>Δ71–110</sub> channels in the absence of point charges and correspond to linear functions between channel conductance and bulk aqueous concentration (eqn. (7); <i>G(c) = G<sub>0</sub>⋅c</i>).</p

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The Francis Crick Institute

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Last time updated on 12/02/2018

This paper was published in The Francis Crick Institute.

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